Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase.
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BORIS DOI
Date of Publication
2022
Publication Type
Article
Division/Institute
Author
Beneke, Tom | |
Dobramysl, Ulrich | |
Catta-Preta, Carolina Moura Costa | |
Mottram, Jeremy Charles | |
Wheeler, Richard J |
Subject(s)
Series
Wellcome open research
ISSN or ISBN (if monograph)
2398-502X
Publisher
Wellcome Trust
Language
English
Publisher DOI
PubMed ID
36874584
Uncontrolled Keywords
Description
We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
File(s)
| File | File Type | Format | Size | License | Publisher/Copright statement | Content | |
|---|---|---|---|---|---|---|---|
| 25e88b6b-2efb-4275-815c-d2558d0f0812_18575_-_richard_wheeler__1_.pdf | text | Adobe PDF | 413.12 KB | Attribution (CC BY 4.0) | published |