Publication:
Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase.

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cris.virtualsource.author-orcid92d768c0-39ab-4a3d-8113-cef40b90b0d3
datacite.rightsopen.access
dc.contributor.authorBeneke, Tom
dc.contributor.authorDobramysl, Ulrich
dc.contributor.authorCatta-Preta, Carolina Moura Costa
dc.contributor.authorMottram, Jeremy Charles
dc.contributor.authorGlünz, Eva Miriam
dc.contributor.authorWheeler, Richard J
dc.date.accessioned2024-10-25T15:47:25Z
dc.date.available2024-10-25T15:47:25Z
dc.date.issued2022
dc.description.abstractWe present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
dc.description.sponsorshipInstitut für Zellbiologie (IZB)
dc.identifier.doi10.48350/179595
dc.identifier.pmid36874584
dc.identifier.publisherDOI10.12688/wellcomeopenres.18575.1
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/164764
dc.language.isoen
dc.publisherWellcome Trust
dc.relation.ispartofWellcome open research
dc.relation.issn2398-502X
dc.relation.organizationDCD5A442C578E17DE0405C82790C4DE2
dc.subjectGenome Leishmania mexicana Pilon polish
dc.subject.ddc500 - Science::570 - Life sciences; biology
dc.titleGenome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase.
dc.typearticle
dspace.entity.typePublication
dspace.file.typetext
oaire.citation.issue294
oaire.citation.startPage294
oaire.citation.volume7
oairecerif.author.affiliationInstitut für Zellbiologie (IZB)
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unibe.date.licenseChanged2023-03-10 05:42:12
unibe.description.ispublishedpub
unibe.eprints.legacyId179595
unibe.refereedtrue
unibe.subtype.articlejournal

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