Occurrence of gustducin-immunoreactive cells in von Ebner's glands of guinea pigs
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BORIS DOI
Date of Publication
2013
Publication Type
Article
Division/Institute
Author
Subject(s)
Series
Histochemistry and cell biology
ISSN or ISBN (if monograph)
0948-6143
Publisher
Springer
Language
English
Publisher DOI
PubMed ID
23604549
Description
An immunohistochemical examination of guinea-pig taste buds in vallate papillae revealed gustducin-immunoreactive cells in the area of von Ebner’s glands, minor salivary glands. Since there have been no reports describing those cells in these locations for other species, we investigated these glands in order both to localize the cells and compare their immunoreactive characteristics with corresponding cells in the vallate taste buds. The gustducin-immunoreactive cells coincided with cells containing no secretory granules in the end portion of the glands, which was supported by the electron-microscopic immunocytochemistry. Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor (IP3R-3), phospholipase Cβ2 (PLCβ2), and villin and also partly immunopositive to neuron-specific enolase (NSE) and calbindin D-28K. The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules. Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in function(s) e.g., chemo-reception.
File(s)
File | File Type | Format | Size | License | Publisher/Copright statement | Content | |
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15149.pdf | text | Adobe PDF | 475.01 KB | Attribution (CC BY 4.0) | published |