New window into hepatitis B in Africa: Liver sampling combined with single cell omics enables deep and longitudinal assessment of intrahepatic immunity in Zambia.
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BORIS DOI
Date of Publication
November 15, 2024
Publication Type
Article
Division/Institute
Contributor
Musonda, Taonga | |
Wallace, Michael S | |
Patel, Hailey | |
Martin, Owen P | |
Oetheimer, Christopher | |
Mwakamui, Simutanyi | |
Sinkala, Edford | |
Nsokolo, Bright | |
Kanunga, Annie | |
Lauer, Georg | |
Chung, Raymond T | |
Bhattacharya, Debika | |
Kelly, Paul | |
Alatrakchi, Nadia | |
Vinikoor, Michael J |
Series
Journal of infectious diseases
ISSN or ISBN (if monograph)
0022-1899
Publisher
Oxford University Press
Language
English
Publisher DOI
PubMed ID
38332750
Uncontrolled Keywords
Description
In Lusaka, Zambia, we introduced liver fine needle aspiration (FNA) into a research cohort of adults with treatment-naïve chronic hepatitis B virus (HBV) infection, with and without HIV coinfection, as well as with acute HBV infection. Over 117 enrollment and 47 longitudinal FNAs (at 1 year follow-up), we established participant acceptability and safety. We also demonstrated the quality of the material through single cell RNA sequencing of selected enrollment FNAs, which revealed a range of immune cells. This approach can drive new insights into HBV immunology, informing cure strategies, and can improve our understanding of HBV natural history in Africa.