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  3. Morphofunctional changes at the active zone during synaptic vesicle exocytosis.
 

Morphofunctional changes at the active zone during synaptic vesicle exocytosis.

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BORIS DOI
10.48350/179590
Date of Publication
May 4, 2023
Publication Type
Article
Division/Institute

Institut für Anatomie...

Author
Radecke, Julikaorcid-logo
Institut für Anatomie
Seeger, Raphaela Isabelle Marianne
Institut für Anatomie
Kádková, Anna
Laugks, Ulrike
Khosrozadeh, Mohammadamin
Institut für Anatomie
Goldie, Kenneth N
Lučić, Vladan
Sørensen, Jakob B
Zuber, Benoîtorcid-logo
Institut für Anatomie
Subject(s)

600 - Technology::610...

Series
EMBO reports
ISSN or ISBN (if monograph)
1469-221X
Publisher
Nature Publishing Group
Language
English
Publisher DOI
10.15252/embr.202255719
PubMed ID
36876590
Uncontrolled Keywords

SNARE cryo-electron t...

Description
Synaptic vesicle (SV) fusion with the plasma membrane (PM) proceeds through intermediate steps that remain poorly resolved. The effect of persistent high or low exocytosis activity on intermediate steps remains unknown. Using spray-mixing plunge-freezing cryo-electron tomography we observe events following synaptic stimulation at nanometer resolution in near-native samples. Our data suggest that during the stage that immediately follows stimulation, termed early fusion, PM and SV membrane curvature changes to establish a point contact. The next stage-late fusion-shows fusion pore opening and SV collapse. During early fusion, proximal tethered SVs form additional tethers with the PM and increase the inter-SV connector number. In the late-fusion stage, PM-proximal SVs lose their interconnections, allowing them to move toward the PM. Two SNAP-25 mutations, one arresting and one disinhibiting spontaneous release, cause connector loss. The disinhibiting mutation causes loss of membrane-proximal multiple-tethered SVs. Overall, tether formation and connector dissolution are triggered by stimulation and respond to spontaneous fusion rate manipulation. These morphological observations likely correspond to SV transition from one functional pool to another.
Handle
https://boris-portal.unibe.ch/handle/20.500.12422/164759
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EMBO_Reports_-_2023_-_Radecke_-_Morphofunctional_changes_at_the_active_zone_during_synaptic_vesicle_exocytosis.pdftextAdobe PDF3.09 MBAttribution (CC BY 4.0)publishedOpen
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