Characterization of miRNA-regulated networks, hubs of signaling, and biomarkers in obstruction-induced bladder dysfunction.
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BORIS DOI
Date of Publication
January 26, 2017
Publication Type
Article
Division/Institute
Author
Keller, Irene | |
Rehrauer, Hubert | |
Fournier, Catharine Aquino |
Subject(s)
Series
JCI insight
ISSN or ISBN (if monograph)
2379-3708
Publisher
JCI Insight
Language
English
Publisher DOI
PubMed ID
28138557
Description
Bladder outlet obstruction (BOO) induces significant organ remodeling, leading to lower urinary tract symptoms accompanied by urodynamic changes in bladder function. Here, we report mRNA and miRNA transcriptome sequencing of bladder samples from human patients with different urodynamically defined states of BOO. Patients' miRNA and mRNA expression profiles correlated with urodynamic findings. Validation of RNA sequencing results in an independent patient cohort identified combinations of 3 mRNAs (NRXN3, BMP7, UPK1A) and 3 miRNAs (miR-103a-3p, miR-10a-5p, miR-199a-3p) sufficient to discriminate between bladder functional states. All BOO patients shared cytokine and immune response pathways, TGF-β and NO signaling pathways, and hypertrophic PI3K/AKT signaling pathways. AP-1 and NFkB were dominant transcription factors, and TNF-α was the top upstream regulator. Integrated miRNA-mRNA expression analysis identified pathways and molecules targeted by differentially expressed miRNAs. Molecular changes in BOO suggest an increasing involvement of miRNAs in the control of bladder function from the overactive to underactive/acontractile states.
File(s)
File | File Type | Format | Size | License | Publisher/Copright statement | Content | |
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JCI Insight89560.pdf | text | Adobe PDF | 3.47 MB | publisher | published |