Publication: Naive B-cell trafficking is shaped by local chemokine availability and LFA-1-independent stromal interactions.
cris.virtualsource.author-orcid | 14788756-7edb-493c-a891-07a20cc1e11e | |
cris.virtualsource.author-orcid | 52a038e5-3a93-434f-aeae-610bf71af3b2 | |
cris.virtualsource.author-orcid | fdd36509-1e9d-4f65-aede-999090f44557 | |
cris.virtualsource.author-orcid | 707c847b-a2b8-4662-8602-d261e3352cdf | |
cris.virtualsource.author-orcid | d921c8ea-e508-41fc-8eb8-4cb615e97237 | |
datacite.rights | open.access | |
dc.contributor.author | Matos Coelho, Fernanda | |
dc.contributor.author | Natale, Daniela | |
dc.contributor.author | Soriano, Silvia F. | |
dc.contributor.author | Hons, Miroslav | |
dc.contributor.author | Swoger, Jim | |
dc.contributor.author | Mayer, Jürgen | |
dc.contributor.author | Danuser, Renzo | |
dc.contributor.author | Scandella, Elke | |
dc.contributor.author | Ackerknecht, Markus | |
dc.contributor.author | Zerwes, Hans-Günter | |
dc.contributor.author | Junt, Tobias | |
dc.contributor.author | Sailer, Andreas W. | |
dc.contributor.author | Ludewig, Burkhard | |
dc.contributor.author | Sharpe, James | |
dc.contributor.author | Figge, Marc Thilo | |
dc.contributor.author | Stein, Jens Volker | |
dc.date.accessioned | 2024-10-15T06:37:27Z | |
dc.date.available | 2024-10-15T06:37:27Z | |
dc.date.issued | 2013-05-16 | |
dc.description.abstract | It is not known how naive B cells compute divergent chemoattractant signals of the T-cell area and B-cell follicles during in vivo migration. Here, we used two-photon microscopy of peripheral lymph nodes (PLNs) to analyze the prototype G-protein-coupled receptors (GPCRs) CXCR4, CXCR5, and CCR7 during B-cell migration, as well as the integrin LFA-1 for stromal guidance. CXCR4 and CCR7 did not influence parenchymal B-cell motility and distribution, despite their role during B-cell arrest in venules. In contrast, CXCR5 played a nonredundant role in B-cell motility in follicles and in the T-cell area. B-cell migration in the T-cell area followed a random guided walk model, arguing against directed migration in vivo. LFA-1, but not α4 integrins, contributed to B-cell motility in PLNs. However, stromal network guidance was LFA-1 independent, uncoupling integrin-dependent migration from stromal attachment. Finally, we observed that despite a 20-fold reduction of chemokine expression in virus-challenged PLNs, CXCR5 remained essential for B-cell screening of antigen-presenting cells. Our data provide an overview of the contribution of prototype GPCRs and integrins during naive B-cell migration and shed light on the local chemokine availability that these cells compute. | |
dc.description.numberOfPages | 9 | |
dc.description.sponsorship | Theodor-Kocher-Institut (TKI) | |
dc.identifier.doi | 10.7892/boris.48613 | |
dc.identifier.pmid | 23558016 | |
dc.identifier.publisherDOI | 10.1182/blood-2012-10-465336 | |
dc.identifier.uri | https://boris-portal.unibe.ch/handle/20.500.12422/119268 | |
dc.language.iso | en | |
dc.publisher | American Society of Hematology | |
dc.relation.ispartof | Blood | |
dc.relation.issn | 0006-4971 | |
dc.relation.organization | DCD5A442BF88E17DE0405C82790C4DE2 | |
dc.subject.ddc | 600 - Technology::610 - Medicine & health | |
dc.title | Naive B-cell trafficking is shaped by local chemokine availability and LFA-1-independent stromal interactions. | |
dc.type | article | |
dspace.entity.type | Publication | |
dspace.file.type | text | |
dspace.file.type | text | |
oaire.citation.endPage | 4109 | |
oaire.citation.issue | 20 | |
oaire.citation.startPage | 4101 | |
oaire.citation.volume | 121 | |
oairecerif.author.affiliation | Theodor-Kocher-Institut (TKI) | |
oairecerif.author.affiliation | Theodor-Kocher-Institut (TKI) | |
oairecerif.author.affiliation | Theodor-Kocher-Institut (TKI) | |
oairecerif.author.affiliation | Theodor-Kocher-Institut (TKI) | |
oairecerif.author.affiliation | Theodor-Kocher-Institut (TKI) | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.contributor.role | creator | |
unibe.description.ispublished | pub | |
unibe.eprints.legacyId | 48613 | |
unibe.journal.abbrevTitle | BLOOD | |
unibe.refereed | true | |
unibe.subtype.article | journal |