Publication:
Naive B-cell trafficking is shaped by local chemokine availability and LFA-1-independent stromal interactions.

cris.virtualsource.author-orcid14788756-7edb-493c-a891-07a20cc1e11e
cris.virtualsource.author-orcid52a038e5-3a93-434f-aeae-610bf71af3b2
cris.virtualsource.author-orcidfdd36509-1e9d-4f65-aede-999090f44557
cris.virtualsource.author-orcid707c847b-a2b8-4662-8602-d261e3352cdf
cris.virtualsource.author-orcidd921c8ea-e508-41fc-8eb8-4cb615e97237
datacite.rightsopen.access
dc.contributor.authorMatos Coelho, Fernanda
dc.contributor.authorNatale, Daniela
dc.contributor.authorSoriano, Silvia F.
dc.contributor.authorHons, Miroslav
dc.contributor.authorSwoger, Jim
dc.contributor.authorMayer, Jürgen
dc.contributor.authorDanuser, Renzo
dc.contributor.authorScandella, Elke
dc.contributor.authorAckerknecht, Markus
dc.contributor.authorZerwes, Hans-Günter
dc.contributor.authorJunt, Tobias
dc.contributor.authorSailer, Andreas W.
dc.contributor.authorLudewig, Burkhard
dc.contributor.authorSharpe, James
dc.contributor.authorFigge, Marc Thilo
dc.contributor.authorStein, Jens Volker
dc.date.accessioned2024-10-15T06:37:27Z
dc.date.available2024-10-15T06:37:27Z
dc.date.issued2013-05-16
dc.description.abstractIt is not known how naive B cells compute divergent chemoattractant signals of the T-cell area and B-cell follicles during in vivo migration. Here, we used two-photon microscopy of peripheral lymph nodes (PLNs) to analyze the prototype G-protein-coupled receptors (GPCRs) CXCR4, CXCR5, and CCR7 during B-cell migration, as well as the integrin LFA-1 for stromal guidance. CXCR4 and CCR7 did not influence parenchymal B-cell motility and distribution, despite their role during B-cell arrest in venules. In contrast, CXCR5 played a nonredundant role in B-cell motility in follicles and in the T-cell area. B-cell migration in the T-cell area followed a random guided walk model, arguing against directed migration in vivo. LFA-1, but not α4 integrins, contributed to B-cell motility in PLNs. However, stromal network guidance was LFA-1 independent, uncoupling integrin-dependent migration from stromal attachment. Finally, we observed that despite a 20-fold reduction of chemokine expression in virus-challenged PLNs, CXCR5 remained essential for B-cell screening of antigen-presenting cells. Our data provide an overview of the contribution of prototype GPCRs and integrins during naive B-cell migration and shed light on the local chemokine availability that these cells compute.
dc.description.numberOfPages9
dc.description.sponsorshipTheodor-Kocher-Institut (TKI)
dc.identifier.doi10.7892/boris.48613
dc.identifier.pmid23558016
dc.identifier.publisherDOI10.1182/blood-2012-10-465336
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/119268
dc.language.isoen
dc.publisherAmerican Society of Hematology
dc.relation.ispartofBlood
dc.relation.issn0006-4971
dc.relation.organizationDCD5A442BF88E17DE0405C82790C4DE2
dc.subject.ddc600 - Technology::610 - Medicine & health
dc.titleNaive B-cell trafficking is shaped by local chemokine availability and LFA-1-independent stromal interactions.
dc.typearticle
dspace.entity.typePublication
dspace.file.typetext
dspace.file.typetext
oaire.citation.endPage4109
oaire.citation.issue20
oaire.citation.startPage4101
oaire.citation.volume121
oairecerif.author.affiliationTheodor-Kocher-Institut (TKI)
oairecerif.author.affiliationTheodor-Kocher-Institut (TKI)
oairecerif.author.affiliationTheodor-Kocher-Institut (TKI)
oairecerif.author.affiliationTheodor-Kocher-Institut (TKI)
oairecerif.author.affiliationTheodor-Kocher-Institut (TKI)
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.contributor.rolecreator
unibe.description.ispublishedpub
unibe.eprints.legacyId48613
unibe.journal.abbrevTitleBLOOD
unibe.refereedtrue
unibe.subtype.articlejournal

Files

Original bundle
Now showing 1 - 2 of 2
Name:
Stein_Blood_2013.pdf
Size:
2.29 MB
Format:
Adobe Portable Document Format
File Type:
text
License:
publisher
Content:
published
Name:
Manuscript_Stein_R2.pdf
Size:
2.46 MB
Format:
Adobe Portable Document Format
File Type:
text
License:
publisher
Content:
accepted

Collections