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  3. Naive B-cell trafficking is shaped by local chemokine availability and LFA-1-independent stromal interactions.
 

Naive B-cell trafficking is shaped by local chemokine availability and LFA-1-independent stromal interactions.

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BORIS DOI
10.7892/boris.48613
Date of Publication
May 16, 2013
Publication Type
Article
Division/Institute

Theodor-Kocher-Instit...

Author
Matos Coelho, Fernanda
Theodor-Kocher-Institut (TKI)
Natale, Daniela
Soriano, Silvia F.
Hons, Miroslav
Theodor-Kocher-Institut (TKI)
Swoger, Jim
Mayer, Jürgen
Danuser, Renzo
Theodor-Kocher-Institut (TKI)
Scandella, Elke
Ackerknecht, Markus
Theodor-Kocher-Institut (TKI)
Zerwes, Hans-Günter
Junt, Tobias
Sailer, Andreas W.
Ludewig, Burkhard
Sharpe, James
Figge, Marc Thilo
Stein, Jens Volker
Theodor-Kocher-Institut (TKI)
Subject(s)

600 - Technology::610...

Series
Blood
ISSN or ISBN (if monograph)
0006-4971
Publisher
American Society of Hematology
Language
English
Publisher DOI
10.1182/blood-2012-10-465336
PubMed ID
23558016
Description
It is not known how naive B cells compute divergent chemoattractant signals of the T-cell area and B-cell follicles during in vivo migration. Here, we used two-photon microscopy of peripheral lymph nodes (PLNs) to analyze the prototype G-protein-coupled receptors (GPCRs) CXCR4, CXCR5, and CCR7 during B-cell migration, as well as the integrin LFA-1 for stromal guidance. CXCR4 and CCR7 did not influence parenchymal B-cell motility and distribution, despite their role during B-cell arrest in venules. In contrast, CXCR5 played a nonredundant role in B-cell motility in follicles and in the T-cell area. B-cell migration in the T-cell area followed a random guided walk model, arguing against directed migration in vivo. LFA-1, but not α4 integrins, contributed to B-cell motility in PLNs. However, stromal network guidance was LFA-1 independent, uncoupling integrin-dependent migration from stromal attachment. Finally, we observed that despite a 20-fold reduction of chemokine expression in virus-challenged PLNs, CXCR5 remained essential for B-cell screening of antigen-presenting cells. Our data provide an overview of the contribution of prototype GPCRs and integrins during naive B-cell migration and shed light on the local chemokine availability that these cells compute.
Handle
https://boris-portal.unibe.ch/handle/20.500.12422/119268
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File(s)
FileFile TypeFormatSizeLicensePublisher/Copright statementContent
Stein_Blood_2013.pdftextAdobe PDF2.29 MBpublished
Manuscript_Stein_R2.pdftextAdobe PDF2.46 MBacceptedOpen
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