Taioqui Pelá, ViníciusViníciusTaioqui PeláNiemeyer, Samira HelenaSamira HelenaNiemeyer0000-0001-9023-908XBaumann, TommyTommyBaumann0000-0002-5950-4137Levy, Flávia MauadFlávia MauadLevyHenrique-Silva, FlávioFlávioHenrique-SilvaLussi, AdrianAdrianLussiSaads Carvalho, ThiagoThiagoSaads Carvalho0000-0002-2435-1169Buzalaf, Marília Afonso RabeloMarília Afonso RabeloBuzalaf2024-10-112024-10-112022-02-15https://boris-portal.unibe.ch/handle/20.500.12422/85487This study evaluated the combination of a sugarcane cystatin (CaneCPI-5) and sodium fluoride (NaF) in acquired pellicle engineering for the prevention of dental erosion in vitro. 75 human enamel specimens were prepared and divided into 5 treatment groups (n = 15/group): Deionized water (Control); Elmex™ (SnCl2/NaF/AmF); 0.1 mg/ml CaneCPI-5; 500 ppm NaF; and CaneCPI-5+NaF (Combination). The specimens were individually treated (200 μl; 2 min; 37 °C), then incubated in human saliva (200 μl; 1 h, at 37 °C) for acquired pellicle formation. Afterwards, the specimens were submitted to an erosive challenge (1% citric acid, pH 3.6, 10 ml, 2 min, 25 °C). This sequence was conducted 5 times. Percentage of surface microhardness change (%SMC), relative surface reflection intensity (rSRI) and calcium released to the citric acid (CR) were measured and analyzed by One-way ANOVA followed by Tukey's test (p<0.05). In general, all the treatments (SnCl2/NaF/AmF, CaneCPI-5, NaF and Combination) significantly protected the enamel when compared the control group. Regarding %SMC and rSRI, the Combination was the most effective treatment, reducing the %SMC significantly (p<0.01) when compared to all the other treatments, although this difference was not significant in the CR analysis. All treatments demonstrated a protective effect on enamel against dental erosion; however the combination of CaneCPI-5 with NaF showed a greater protection.en600 - Technology::610 - Medicine & health500 - Science::570 - Life sciences; biologyarticle10.48350/1705153516823610.1159/000522490