Roura Padrosa, DavidDavidRoura Padrosa0000-0001-6324-8722Benitez Mateos, Ana IsabelAna IsabelBenitez MateosCalvey, LiamLiamCalveyParadisi, FrancescaFrancescaParadisi0000-0003-1704-06422024-09-022024-09-022020-07https://boris-portal.unibe.ch/handle/20.500.12422/36547As an alternative to the traditional chemical synthesis or in-vivo production of L-Pipecolic acid, we have developed two ex-vivo strategies using purified and immobilised enzymes for the production of this key building block. Firstly, a transaminase capable of lysine ε-deamination was coupled with a novel pyrroline-5-carboxylate reductase, yielding 60% conversion at the 50 mM scale with free enzymes and in-situ recycling of the cofactor. A second, simpler, redox neutral system was then constructed by combining the pyrroline-5-carboxylate reductase with a lysine-6-dehydrogenase. This bienzymatic system, with catalytic amount of free cofactor yielded >99% of pipecolic acid in batch and, following co-immobilisation of both enzymes, it was applied as a packed-bed reactor in continuous flow achieving again a molar conversion of >99% with 30 min residence time, and a space-time yield up to 2.5 g/L/h. The sustainability of the system was further improved by a catch-and-release strategy to purify the product, and recovery and recycling of the cofactor.en500 - Science::540 - Chemistryarticle10.7892/boris.14531910.1039/D0GC01817A