Zuber, BenoîtBenoîtZuber0000-0001-7725-5579Lučić, VladanVladanLučić2024-10-092024-10-092022https://boris-portal.unibe.ch/handle/20.500.12422/69146Cryo-electron tomography (Cryo-ET) provides unique opportunities to image cellular components at high resolution in their native state and environment. While many different cell types were investigated by cryo-ET, here we review application to neurons. We show that neurons are a versatile system that can be used to investigate general cellular components such as the cytoskeleton and membrane-bound organelles, in addition to neuron-specific processes such as synaptic transmission. Furthermore, the synapse provides a rich environment for the development of cryo-ET image processing tools suitable to elucidate the functional and spatial organization of compositionally and morphologically heterogeneous macromolecular complexes involved in biochemical signaling cascades, within their native, crowded cellular environments.enCryo-electron tomography Electron microscopy Neurons Synapses600 - Technology::610 - Medicine & healtharticle10.48350/1678523531040710.1016/j.yjsbx.2022.100067