van Breugel, MarkMarkvan BreugelHirono, MasafumiMasafumiHironoAndreeva, AntoninaAntoninaAndreevaYanagisawa, Haru-akiHaru-akiYanagisawaYamaguchi, ShokoShokoYamaguchiNakazawa, YukiYukiNakazawaMorgner, NinaNinaMorgnerPetrovich, MirianaMirianaPetrovichEbong, Ima-ObongIma-ObongEbongRobinson, Carol VCarol VRobinsonJohnson, Christopher MChristopher MJohnsonVeprintsev, DmitryDmitryVeprintsevZuber, BenoîtBenoîtZuber0000-0001-7725-55792024-10-102024-10-102011https://boris-portal.unibe.ch/handle/20.500.12422/74760Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.enarticle2127344700028797120004310.1126/science.1199325