Rompf, Johanna-RaffaelaJohanna-RaffaelaRompfHettlich, Bianca FelicitasBianca FelicitasHettlichLutz, Bérénice Antonia MathildeBérénice Antonia MathildeLutzMarti, Eliane IsabelleEliane IsabelleMarti0000-0003-1284-4350Mirkovitch, JelenaJelenaMirkovitchPeters, Laureen MichèleLaureen MichèlePetersAdamik, Katja-NicoleKatja-NicoleAdamikSchüpbach, Gertraud IreneGertraud IreneSchüpbachWilli, BarbaraBarbaraWilliSchuller, SimoneSimoneSchuller0000-0002-7316-44232024-10-252024-10-252023-06https://boris-portal.unibe.ch/handle/20.500.12422/165017BACKGROUND Procalcitonin (PCT) is a well-established biomarker for bacterial infection in human patients. OBJECTIVES We aimed to analyze the kinetics of plasma PCT (pPCT) in healthy dogs and dogs with canine cranial cruciate ligament (CCL) rupture undergoing tibial plateau leveling osteotomy (TPLO). METHODS This prospective, longitudinal study included 15 healthy dogs and 25 dogs undergoing TPLO. Hematology, pPCT, and C-reactive protein (CRP) were assessed on 3 consecutive days in healthy dogs and 1 day preoperatively and days 1, 2, 10, and 56 postoperatively. Inter- and intraindividual variability of pPCT were assessed in healthy dogs. Median pPCT concentrations of dogs with CCL rupture preoperatively were compared with healthy controls, and median pPCT concentrations, as well as percentage change post anesthesia, arthroscopy, and TPLO, were compared with baseline. For the correlation analysis, the Spearman rank correlation test was used. RESULTS Inter- and intraindividual variabilities of pPCT in healthy dogs were 36% and 15%, respectively. Median baseline pPCT concentrations were not significantly different between healthy dogs (118.9 pg/mL; IQR: 75.3-157.3 pg/mL) and dogs undergoing TPLO (95.9 pg/mL; IQR: 63.8-117.0 pg/mL). Plasma PCT concentrations were significantly lower immediately post- than preoperatively (P < 0.001). CRP, WBC, and neutrophil concentrations increased significantly on post-OP day 2 and had normalized by day 10. CONCLUSIONS These results indicate that CCL rupture, as well as anesthesia, arthroscopy, and TPLO combined, are not associated with increased pPCT concentrations in dogs with uncomplicated recovery. Considering the high intraindividual variability, individual serial measurements rather than a population-based reference interval should be considered.encanine interindividual variability intraindividual variability orthopedic surgery surgical site infection600 - Technology::630 - Agriculturearticle10.48350/1799053689867810.1111/vcp.13212