Chortarea, SavvinaSavvinaChortareaFytianos, KleanthisKleanthisFytianosRodriguez-Lorenzo, LauraLauraRodriguez-LorenzoPetri-Fink, AlkeAlkePetri-FinkRothen-Rutishauser, BarbaraBarbaraRothen-Rutishauser2024-10-072024-10-072018-05https://boris-portal.unibe.ch/handle/20.500.12422/62072AIM The distribution and impact of aerosol-delivered gold nanoparticles (AuNPs) functionalized with a mixture of aminated-polyvinyl alcohol and amino-PEG ([polyvinyl alcohol/PEG]-NH) upon repeated administration onto a 3D lung model were explored. MATERIALS & METHODS AuNPs were aerosolized and uptake and epithelial translocation was assessed by inductively coupled plasma optical-emission spectroscopy, flow cytometry and electron microscopy. In addition, cytotoxicity, apoptosis and proinflammation were evaluated. RESULTS Repeated AuNP aerosolization resulted in NP accumulation in macrophages and epithelial cells. Dendritic cells demonstrated substantial NP internalization after single administration which was reduced in later time points. No cytotoxicity or proinflammation was observed but after 96 h significant apoptosis was induced by the polymer coating. CONCLUSION These results indicate the importance of repeated exposures in addressing potential effects of NPs.enair–liquid interface biodistribution biomedical gold nanoparticles hazard assessment in vitro 3D lung system short-term repeated aerosol exposure600 - Technology::610 - Medicine & healtharticle10.7892/boris.1232232987414510.2217/nnm-2017-0358