Amort, MelanieMelanieAmortNachbauer, BirgitBirgitNachbauerTuzlak, SelmaSelmaTuzlakKieser, ArndArndKieserSchepers, AloysAloysSchepersVillunger, AndreasAndreasVillungerPolacek, NorbertNorbertPolacek0000-0001-5317-39902024-10-232024-10-232015-05-08https://boris-portal.unibe.ch/handle/20.500.12422/133338Non-protein-coding RNAs are a functionally versatile class of transcripts exerting their biological roles on the RNA level. Recently, we demonstrated that the vault complex-associated RNAs (vtRNAs) are significantly upregulated in Epstein-Barr virus (EBV)-infected human B cells. Very little is known about the function(s) of the vtRNAs or the vault complex. Here, we individually express latent EBV-encoded proteins in B cells and identify the latent membrane protein 1 (LMP1) as trigger for vtRNA upregulation. Ectopic expression of vtRNA1-1, but not of the other vtRNA paralogues, results in an improved viral establishment and reduced apoptosis, a function located in the central domain of vtRNA1-1. Knockdown of the major vault protein has no effect on these phenotypes revealing that vtRNA1-1 and not the vault complex contributes to general cell death resistance. This study describes a NF-κB-mediated role of the non-coding vtRNA1-1 in inhibiting both the extrinsic and intrinsic apoptotic pathways.en500 - Science::570 - Life sciences; biology500 - Science::540 - Chemistryarticle10.7892/boris.6890210.1038/ncomms8030