Diserens, GaëlleGaëlleDiserensVermathen, MartinaMartinaVermathenGjuroski, IlcheIlcheGjuroskiEggimann, SandraSandraEggimannPrecht, Maria ChristinaMaria ChristinaPrecht0000-0001-5415-3780Boesch, Christoph HansChristoph HansBoesch0000-0001-8498-8543Vermathen, PeterPeterVermathen0000-0001-8649-10982025-01-082025-01-082016https://boris-portal.unibe.ch/handle/20.500.12422/198820The study aim was to unambiguously assign nucleotide sugars, mainly UDP-X that are known to be important in glycosylation processes as sugar donors, and glucose-phosphates that are important intermediate metabolites for storage and transfer of energy directly in spectra of intact cells, as well as in skeletal muscle biopsies by 1H high-resolution magic-angle-spinning (HR-MAS) NMR. The results demonstrate that sugar phosphates can be determined quickly and non-destructively in cells and biopsies by HR-MAS, which may prove valuable considering the importance of phosphate sugars in cell metabolism for nucleic acid synthesis. As proof of principle, an example of phosphate-sugar reaction and degradation kinetics after unfreezing the sample is shown for a cardiac muscle, suggesting the possibility to follow by HR-MAS NMR some metabolic pathways.en500 - Science::570 - Life sciences; biology500 - Science::540 - Chemistry600 - Technology::630 - Agriculturearticle10.7892/boris.924902727126110.1007/s00216-016-9671-0