Milia, EgleEgleMiliaUsai, MariannaMariannaUsaiSzotáková, BarboraBarboraSzotákováElstnerová, MarieMarieElstnerováKrálová, VěraVěraKrálováD'hallewin, GuyGuyD'hallewinSpissu, YleniaYleniaSpissuBarberis, AntonioAntonioBarberisMarchetti, MauroMauroMarchettiBortone, AntonellaAntonellaBortoneCampanella, VincenzoVincenzoCampanellaMastandrea, GiorgioGiorgioMastandreaLanghansová, LenkaLenkaLanghansováEick, SigrunSigrunEick2024-09-022024-09-022020-05-26https://boris-portal.unibe.ch/handle/20.500.12422/38263BACKGROUND Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates sp., together with its anti-inflammatory activity and safety. METHODS PLL-EO was screened by gas chromatography/mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX-1/2) and lipoxygenase (LOX) inhibition, while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four lines of oral cells. RESULTS According to the concentrations of terpens, PLL-EO is a pharmacologically-active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. they were between 6.25 and 12.5 µg/mL. Oxidation by COX-1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil, respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose. CONCLUSIONS PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida, with an interesting dual inhibitory capacity toward COX-2 and LOX inflammatory enzymes, and without side effects against oral cells.enCandida albicans Candida glabrata Oral health care products cyclooxygenase lipoxygenase medicinal herbs periodontal disease600 - Technology::610 - Medicine & healtharticle10.7892/boris.1487713246637110.3390/antibiotics9060281