Khoury, PaneezPaneezKhouryAkuthota, PraveenPraveenAkuthotaAckerman, Steven JSteven JAckermanArron, Joseph RJoseph RArronBochner, Bruce SBruce SBochnerCollins, Margaret HMargaret HCollinsKahn, Jean-EmmanuelJean-EmmanuelKahnFulkerson, Patricia CPatricia CFulkersonGleich, Gerald JGerald JGleichGopal-Srivastava, RashmiRashmiGopal-SrivastavaJacobsen, Elizabeth AElizabeth AJacobsenLeiferman, Kristen MKristen MLeifermanFrancesca, Levi-SchafferLevi-SchafferFrancescaMathur, Sameer KSameer KMathurMinnicozzi, MichaelMichaelMinnicozziPrussin, CalmanCalmanPrussinRothenberg, Marc EMarc ERothenbergRoufosse, FlorenceFlorenceRoufosseSable, KathleenKathleenSableSimon, DagmarDagmarSimonSimon, Hans-UweHans-UweSimon0000-0002-9404-7736Spencer, Lisa ALisa ASpencerSteinfeld, JonathanJonathanSteinfeldWardlaw, Andrew JAndrew JWardlawWechsler, Michael EMichael EWechslerWeller, Peter FPeter FWellerKlion, Amy DAmy DKlion2024-10-252024-10-252018-07https://boris-portal.unibe.ch/handle/20.500.12422/162244BACKGROUND Eosinophil-associated diseases (EADs) are rare, heterogeneous disorders characterized by the presence of eosinophils in tissues and/or peripheral blood resulting in immunopathology. The heterogeneity of tissue involvement, lack of sufficient animal models, technical challenges in working with eosinophils, and lack of standardized histopathologic approaches have hampered progress in basic research. Additionally, clinical trials and drug development for rare EADs are limited by the lack of primary and surrogate endpoints, biomarkers, and validated patient-reported outcomes. METHODS Researchers with expertise in eosinophil biology and eosinophil-related diseases reviewed the state of current eosinophil research, resources, progress, and unmet needs in the field since the 2012 meeting of the NIH Taskforce on the Research of Eosinophil-Associated Diseases (TREAD). RESULTS RE-TREAD focused on gaps in basic science, translational, and clinical research on eosinophils and eosinophil-related pathogenesis. Improved recapitulation of human eosinophil biology and pathogenesis in murine models was felt to be of importance. Characterization of eosinophil phenotypes, the role of eosinophil subsets in tissues, identification of biomarkers of eosinophil activation and tissue load, and a better understanding of the role of eosinophils in human disease were prioritized. Finally, an unmet need for tools for use in clinical trials was emphasized. Histopathologic scoring, patient- and clinician-reported outcomes, and appropriate coding were deemed of paramount importance for research collaborations, drug development, and approval by regulatory agencies. Further exploration of the eosinophil genome, epigenome, and proteome was also encouraged. CONCLUSIONS Although progress has been made since 2012, unmet needs in eosinophil research remain a priority.enbiomarkers eosinophil-related disorders eosinophilia hypereosinophilic syndromes murine models translational research600 - Technology::610 - Medicine & healtharticle10.7892/boris.1169032967291410.1002/JLB.5MR0118-028R