Riedel, RenéRenéRiedelAddo, RichardRichardAddoFerreira-Gomes, MartaMartaFerreira-GomesHeinz, Gitta AnneGitta AnneHeinzHeinrich, FrederikFrederikHeinrichKummer, JannisJannisKummerGreiff, VictorVictorGreiffSchulz, DanielDanielSchulzKlaeden, CoraCoraKlaedenCornelis, RebeccaRebeccaCornelisMenzel, UlrikeUlrikeMenzelKröger, StefanStefanKrögerStervbo, UlrikUlrikStervboKöhler, RalfRalfKöhlerHaftmann, ClaudiaClaudiaHaftmannKühnel, SilviaSilviaKühnelLehmann, KatrinKatrinLehmannMaschmeyer, PatrickPatrickMaschmeyerMcGrath, MairiMairiMcGrathNaundorf, SandraSandraNaundorfHahne, StefanieStefanieHahneSercan-Alp, ÖzenÖzenSercan-AlpSiracusa, FrancescoFrancescoSiracusaStefanowski, JonathanJonathanStefanowskiWeber, MelanieMelanieWeberWestendorf, KerstinKerstinWestendorfZimmermann, JakobJakobZimmermann0000-0002-6302-6844Hauser, Anja EAnja EHauserReddy, Sai TSai TReddyDurek, PawelPawelDurekChang, Hyun-DongHyun-DongChangMashreghi, Mir-FarzinMir-FarzinMashreghiRadbruch, AndreasAndreasRadbruch2024-09-022024-09-022020-05-22https://boris-portal.unibe.ch/handle/20.500.12422/38837At present, it is not clear how memory B lymphocytes are maintained over time, and whether only as circulating cells or also residing in particular tissues. Here we describe distinct populations of isotype-switched memory B lymphocytes (Bsm) of murine spleen and bone marrow, identified according to individual transcriptional signature and B cell receptor repertoire. A population of marginal zone-like cells is located exclusively in the spleen, while a population of quiescent Bsm is found only in the bone marrow. Three further resident populations, present in spleen and bone marrow, represent transitional and follicular B cells and B1 cells, respectively. A population representing 10-20% of spleen and bone marrow memory B cells is the only one qualifying as circulating. In the bone marrow, all cells individually dock onto VCAM1+ stromal cells and, reminiscent of resident memory T and plasma cells, are void of activation, proliferation and mobility.en600 - Technology::610 - Medicine & healtharticle10.7892/boris.1497403244463110.1038/s41467-020-16464-6