Ricardo-Lax, InnaInnaRicardo-LaxLuna, Joseph MJoseph MLunaTran, Thi Nhu ThaoThi Nhu ThaoTran0000-0003-3906-4629Le Pen, JérémieJérémieLe PenYu, YingpuYingpuYuHoffmann, H-HeinrichH-HeinrichHoffmannSchneider, William MWilliam MSchneiderRazooky, Brandon SBrandon SRazookyFernandez-Martinez, JavierJavierFernandez-MartinezSchmidt, FabianFabianSchmidtWeisblum, YiskaYiskaWeisblumTrüeb, Bettina SalomeBettina SalomeTrüebBerenguer Veiga, Inês MargaridaInês MargaridaBerenguer Veiga0000-0001-8554-790XSchmied, KimberlyKimberlySchmiedEbert, NadineNadineEbertMichailidis, EleftheriosEleftheriosMichailidisPeace, AveryAveryPeaceSánchez-Rivera, Francisco JFrancisco JSánchez-RiveraLowe, Scott WScott WLoweRout, Michael PMichael PRoutHatziioannou, TheodoraTheodoraHatziioannouBieniasz, Paul DPaul DBieniaszPoirier, John TJohn TPoirierMacDonald, Margaret RMargaret RMacDonaldThiel, Volker EarlVolker EarlThielRice, Charles MCharles MRice2024-10-092024-10-092021-11-26https://boris-portal.unibe.ch/handle/20.500.12422/67537Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.en500 - Science500 - Science::570 - Life sciences; biology500 - Science::590 - Animals (Zoology)600 - Technology::610 - Medicine & health600 - Technology::630 - Agriculturearticle10.48350/1656323464837110.1126/science.abj8430