García-Poyatos, CarolinaCarolinaGarcía-PoyatosArora, PrateekPrateekAroraCalvo, EnriqueEnriqueCalvodos Santos Marques, Ines JoaoInes Joaodos Santos MarquesKirschke, NickNickKirschkeGalardi-Castilla, MariaMariaGalardi-CastillaLembke, Carla-SophieCarla-SophieLembkeMeer, Marco DanielMarco DanielMeerFernández-Montes, PaulaPaulaFernández-MontesErnst, Alexander Uwe JohannAlexander Uwe JohannErnst0000-0002-4956-315XHaberthür, DavidDavidHaberthür0000-0003-3388-9187Hlushchuk, RuslanRuslanHlushchukVázquez, JesúsJesúsVázquezVermathen, PeterPeterVermathen0000-0001-8649-1098Enríquez, José AntonioJosé AntonioEnríquezMercader Huber, Nadia IsabelNadia IsabelMercader Huber0000-0002-0905-63992024-10-262024-10-262024-07-22https://boris-portal.unibe.ch/handle/20.500.12422/177198The oxidative phosphorylation (OXPHOS) system is intricately organized, with respiratory complexes forming super-assembled quaternary structures whose assembly mechanisms and physiological roles remain under investigation. Cox7a2l, also known as Scaf1, facilitates complex III and complex IV (CIII-CIV) super-assembly, enhancing energetic efficiency in various species. We examined the role of Cox7a1, another Cox7a family member, in supercomplex assembly and muscle physiology. Zebrafish lacking Cox7a1 exhibited reduced CIV2 formation, metabolic alterations, and non-pathological muscle performance decline. Additionally, cox7a1-/- hearts displayed a pro-regenerative metabolic profile, impacting cardiac regenerative response. The distinct phenotypic effects of cox7a1-/- and cox7a2l-/- underscore the diverse metabolic and physiological consequences of impaired supercomplex formation, emphasizing the significance of Cox7a1 in muscle maturation within the OXPHOS system.enCa(2+) signaling cox7a1 cox7a2l electron transport chain heart regeneration metabolic rewiring mitochondria muscle physiology scaf1 supercomplex assembly zebrafish600 - Technology::610 - Medicine & health500 - Science::570 - Life sciences; biologyarticle10.48350/1965243870178410.1016/j.devcel.2024.04.012