Roukens, Jaap-JanJaap-JanRoukensGertsch, JürgJürgGertschMeier, PhilipPhilipMeier2025-11-172025-11-10https://boris-portal.unibe.ch/handle/20.500.12422/223532This .txt file was generated on 2025.11.14 by Jaap-Jan Roukens. GENERAL INFORMATION 1. Title of dataset: Supplementary figures for illuminating chromaffin granules compartmentalization of endocannabinoids in bovine adrenal glands supports role in hormone modulation 2. Creator information: Principal Investigator / Corresponding Author: Name: Jürg Gertsch Institution: University of Bern, Institute of Biochemistry and Molecular Medicine Email: juerg.gertsch@unibe.ch Data Creator / Submitter: Name: Jaap-Jan Roukens Institution: University of Bern, Institute of Biochemistry and Molecular Medicine Other Creators: Name: Philip Meier Institution: University of Bern, Institute of Biochemistry and Molecular Medicine 3. Date of data collection: Data was generated over the course of the study (approx. 2023-2025). See associated publication for methodological timeline. 4. Geographic location of data collection: Bovine adrenal glands were obtained from a local abattoir (Bell Switzerland AG, Oensingen, Switzerland). 5. Keywords describing the subject of your dataset: Endocannabinoid system, peptide hormone, adrenal glands, RVD-hemopressin, reserpine, VMAT2. 6. Information about funding sources that supported the collection of the data: This work was supported by the Swiss National Science Foundation, grant No 189220. --- ACCESS INFORMATION 1. Access restrictions: None. This dataset is openly available. 2. License: Creative Commons Attribution 4.0 International (CC-BY 4.0) 3. Links/relationships to additional data sets: This dataset provides supplementary figures (S1-S6) for the main article: "Illuminating chromaffin granules: Compartmentalization of endocannabinoids in bovine adrenal glands supports role in hormone modulation". Main article DOI: [To be added upon publication] 4. Was data derived from another source? No, this is original data generated for the associated study. 5. Recommended citation for this dataset: Roukens, Jaap-Jan; Meier, Philip; Simão, Ana Catarina; Chicca, Andrea; Bregy, Rachel; Altmann, Karl-Heinz; Gertsch, Jürg. (2025). Supplementary figures for illuminating chromaffin granules compartmentalization of endocannabinoids in bovine adrenal glands supports role in hormone modulation [Dataset]. Bern: University of Bern, BORIS Portal. [https://doi.org/10.48620/92152] --- DATA & FILE OVERVIEW 1. Filenames & short description: * Supplementary_Figures_Roukens2025.pdf: All supplementary figures (S1-S6) combined in a single PDF file. * S1.TIF: VMAT2-targeted staining in live PC12 cells using probe CV-28. * S2.TIF: Representative cryo-electron micrograph of isolated bovine chromaffin granules. * S3.TIF: Flow cytometry analysis of chromaffin granules and artificial liposomes. * S4.TIF: Full uncropped ABPP gels for NCI-H295R cells and mouse brain membrane preparations. * S5.TIF: 2-Arachidonoylglycerol (2-AG) does not modulate dopamine secretion in PC12 cells. * S6.TIF: Proton (1H) NMR spectrum (400 MHz) of CV-28. 2. File format: .pdf, .jpg (or .tiff) and .txt 3. Date of creation: 20251114 4. Are there multiple versions of the dataset? Yes. This is version 2 (v.2), uploaded on 2025.11.14. This version supersedes v.1 (uploaded 2025.11.06). This update was made to remove supplementary files that during revisions were moved to the main manuscript. --- METHODOLOGICAL INFORMATION 1. Description of methods for collection/generation of data: The methods for data collection, processing, and quality assurance are described in detail in the '2. Materials and Methods' section of the associated main article (DOI pending) and in the captions of the Supplementary Figures file .enEndocannabinoid systempeptide hormoneadrenal glandsRVD-hemopressinreserpineVMAT2Large dense-core vesicles500 - Science::570 - Life sciences; biologydataset10.48620/92409