Arfman, TomTomArfmanZollet, ValentinaValentinaZolletvan Es, NickNickvan EsBosch, Floris T MFloris T MBoschNicolaes, Gerry A FGerry A FNicolaesSorvillo, NicolettaNicolettaSorvilloVoorberg, JanJanVoorberg2024-10-262024-10-262024-02https://boris-portal.unibe.ch/handle/20.500.12422/174731Neutrophil released peptidyl arginine deiminase 4 (PAD4) converts arginine residues on plasma proteins into citrulline. Here, we developed an assay to quantify citrullinated fibrinogen. We employed a biotin-conjugated phenylglyoxal (biotin-phenylglyoxal (PG)) compound that selectively labels citrulline. Patient samples were derived from a multicenter prospective cohort study that aimed to identify cancer patients at high risk for venous thromboembolism (VTE). Our data show that cancer patients have higher (median 2-fold increased) citrullinated fibrinogen levels when compared to normal human plasma and a cohort of healthy donors. Our results show that citrullination of fibrinogen is a common posttranslational modification in patients with cancer.encancer citrullination coagulation fibrinogen thrombosis500 - Science::570 - Life sciences; biology600 - Technology::610 - Medicine & healtharticle10.48350/1932703840651010.1002/jha2.825