Harder, DanielDanielHarderRitzmann, NoahNoahRitzmannUcurum Fotiadis, ZöhreZöhreUcurum FotiadisMüller, Daniel JDaniel JMüllerFotiadis, Dimitrios JoséDimitrios JoséFotiadis2024-10-262024-10-262024-04https://boris-portal.unibe.ch/handle/20.500.12422/174355Controlling the pH at the microliter scale can be useful for applications in research, medicine, and industry, and therefore represents a valuable application for synthetic biology and microfluidics. The presented vesicular system translates light of different colors into specific pH changes in the surrounding solution. It works with the two light-driven proton pumps bacteriorhodopsin and blue light-absorbing proteorhodopsin Med12, that are oriented in opposite directions in the lipid membrane. A computer-controlled measuring device implements a feedback loop for automatic adjustment and maintenance of a selected pH value. A pH range spanning more than two units can be established, providing fine temporal and pH resolution. As an application example, a pH-sensitive enzyme reaction is presented where the light color controls the reaction progress. In summary, light color-controlled pH-adjustment using engineered proteoliposomes opens new possibilities to control processes at the microliter scale in different contexts, such as in synthetic biology applications.enbacteriorhodopsin light-driven proton pump proteoliposome proteorhodopsin synthetic biology500 - Science::570 - Life sciences; biology600 - Technology::610 - Medicine & healtharticle10.48350/1928123834261810.1002/advs.202307524