Lang, Niklas JNiklas JLangGote-Schniering, JanineJanineGote-SchnieringPorras-Gonzalez, DianaDianaPorras-GonzalezYang, LinLinYangDe Sadeleer, Laurens JLaurens JDe SadeleerJentzsch, R ChristophR ChristophJentzschShitov, Vladimir AVladimir AShitovZhou, ShuhongShuhongZhouAnsari, MeshalMeshalAnsariAgami, AhmedAhmedAgamiMayr, Christoph HChristoph HMayrHooshiar Kashani, BaharakBaharakHooshiar KashaniChen, YuexinYuexinChenHeumos, LukasLukasHeumosPestoni, Jeanine CJeanine CPestoniMolnar, Eszter SaroltaEszter SaroltaMolnarGeeraerts, EmielEmielGeeraertsAnquetil, VincentVincentAnquetilSaniere, LaurentLaurentSaniereWögrath, MelanieMelanieWögrathGerckens, MichaelMichaelGerckensLehmann, MareikeMareikeLehmannYildirim, Ali ÖnderAli ÖnderYildirimHatz, RudolfRudolfHatzKneidinger, NikolausNikolausKneidingerBehr, JürgenJürgenBehrWuyts, Wim AWim AWuytsStoleriu, Mircea-GabrielMircea-GabrielStoleriuLuecken, Malte DMalte DLueckenTheis, Fabian JFabian JTheisBurgstaller, GeraldGeraldBurgstallerSchiller, Herbert BHerbert BSchiller2024-10-252024-10-252023-12-06https://boris-portal.unibe.ch/handle/20.500.12422/172075Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing mechanisms of regeneration and fibrogenesis directly in human tissue has been hampered by the lack of organotypic models and analytical techniques. In this work, we coupled ex vivo cytokine and drug perturbations of human precision-cut lung slices (hPCLS) with single-cell RNA sequencing and induced a multilineage circuit of fibrogenic cell states in hPCLS. We showed that these cell states were highly similar to the in vivo cell circuit in a multicohort lung cell atlas from patients with pulmonary fibrosis. Using micro-CT-staged patient tissues, we characterized the appearance and interaction of myofibroblasts, an ectopic endothelial cell state, and basaloid epithelial cells in the thickened alveolar septum of early-stage lung fibrosis. Induction of these states in the hPCLS model provided evidence that the basaloid cell state was derived from alveolar type 2 cells, whereas the ectopic endothelial cell state emerged from capillary cell plasticity. Cell-cell communication routes in patients were largely conserved in hPCLS, and antifibrotic drug treatments showed highly cell type-specific effects. Our work provides an experimental framework for perturbational single-cell genomics directly in human lung tissue that enables analysis of tissue homeostasis, regeneration, and pathology. We further demonstrate that hPCLS offer an avenue for scalable, high-resolution drug testing to accelerate antifibrotic drug development and translation.en600 - Technology::610 - Medicine & healtharticle10.48350/1899033805580310.1126/scitranslmed.adh0908