Hillenbrand, Caroline ACaroline AHillenbrandBani, Dorssa AkbariDorssa AkbariBaniFollonier, OcéaneOcéaneFollonierKaur, AmandeepAmandeepKaurWeissbach, Fabian HFabian HWeissbachWernli, MarionMarionWernliWilhelm, MaudMaudWilhelmLeuzinger, KarolineKarolineLeuzingerBinet, IsabelleIsabelleBinetBochud, Pierre-YvesPierre-YvesBochudGolshayan, DelaDelaGolshayanHirzel, CédricCédricHirzel0000-0002-7870-912XManuel, OriolOriolManuelMueller, Nicolas JNicolas JMuellerSchaub, StefanStefanSchaubSchachtner, ThomasThomasSchachtnerVan Delden, ChristianChristianVan DeldenHirsch, Hans HHans HHirsch2024-12-112024-12-112025-05https://boris-portal.unibe.ch/handle/20.500.12422/191413Collaborators "Swiss Transplant Cohort Study": Annalisa Berzigotti, Guido Stirnimann , Vanessa Banz, Guido Beldi (UVCM Department of Visceral Surgery and Medicine).BK polyomavirus (BKPyV) causes premature renal failure in 10%-30% of kidney transplant recipients (KTRs). Current guidelines recommend screening for new-onset BKPyV-DNAemia/nephropathy and reducing immunosuppression to regain BKPyV-specific immune control. Since BKPyV encompasses 4 major genotype(gt)-encoded serotypes (st1,-2,-3,-4), st-specific antibodies may inform risk and course of BKPyV-DNAemia/nephropathy. Using BKPyV st-virus-like particle (VLP) ELISA, we analyzed plasma from 399 blood donors (BDs) and 428 KTRs (134 KTR-cases with BKPyV-DNAemia, 294 KTR-controls). BDs were anti-BKPyV-VLP IgG-seropositive in 85% compared to 93% of KTRs at T0 (p<0.001). Anti-st1 were predominant in both groups followed by anti-st4, anti-st2, and anti-st3. Antibody levels and quadruple sero-reactivity at T0 were higher in KTR-controls than in KTR-cases (p=0.026) or in BDs (p<0.001). In KTR-cases, anti-st increased post-transplant (p<0.0001) and independently of ongoing or cleared BKPyV-DNAemia. However, anti-st levels were significantly higher at T0 in KTR-cases able to clear at T6 or T12. In 34 KTR-cases with deep genome sequencing, BKPyV-gtI was predominant, and anti-st1 and st1-neutralizing antibodies were significantly lower at T0 than in KTR-controls. Thus, BKPyV st-specific antibody levels at transplantation might reflect gt/st-BKPyV-specific immunity clearing or preventing BKPyV-DNAemia in KTR-cases or KTR-controls, respectively. Accordingly, active or passive immunization may be most efficient pretransplant or early post-transplant.enBK virusKidneyantibodyblood donorsintravenous immunoglobulinneutralizationpolyomaserologytransplantation600 - Technology::610 - Medicine & healtharticle10.48620/773593958007510.1016/j.ajt.2024.11.019