• LOGIN
Repository logo

BORIS Portal

Bern Open Repository and Information System

  • Publication
  • Projects
  • Funding
  • Research Data
  • Organizations
  • Researchers
  • LOGIN
Repository logo
Unibern.ch
  1. Home
  2. Publications
  3. Ultrasensitive Label-Free Detection of Protein-Membrane Interaction Exemplified by Toxin-Liposome Insertion.
 

Ultrasensitive Label-Free Detection of Protein-Membrane Interaction Exemplified by Toxin-Liposome Insertion.

Options
  • Details
BORIS DOI
10.48350/169000
Date of Publication
April 14, 2022
Publication Type
Article
Division/Institute

Institut für Anatomie...

Author
Schönfeldová, T
Okur, H I
Vezočnik, V
Iacovache, Mircea Ioan
Institut für Anatomie
Cao, C
Dal Peraro, M
Maček, P
Zuber, Benoîtorcid-logo
Institut für Anatomie
Roke, S
Subject(s)

600 - Technology::610...

Series
The journal of physical chemistry letters
ISSN or ISBN (if monograph)
1948-7185
Publisher
American Chemical Society
Language
English
Publisher DOI
10.1021/acs.jpclett.1c04011
PubMed ID
35377651
Description
Measuring the high-affinity binding of proteins to liposome membranes remains a challenge. Here, we show an ultrasensitive and direct detection of protein binding to liposome membranes using high throughput second harmonic scattering (SHS). Perfringolysin O (PFO), a pore-forming toxin, with a highly membrane selective insertion into cholesterol-rich membranes is used. PFO inserts only into liposomes with a cholesterol concentration >30%. Twenty mole-percent cholesterol results in neither SHS-signal deviation nor pore formation as seen by cryo-electron microscopy of PFO and liposomes. PFO inserts into cholesterol-rich membranes of large unilamellar vesicles in an aqueous solution with Kd = (1.5 ± 0.2) × 10-12 M. Our results demonstrate a promising approach to probe protein-membrane interactions below sub-picomolar concentrations in a label-free and noninvasive manner on 3D systems. More importantly, the volume of protein sample is ultrasmall (<10 μL). These findings enable the detection of low-abundance proteins and their interaction with membranes.
Handle
https://boris-portal.unibe.ch/handle/20.500.12422/69940
Show full item
File(s)
FileFile TypeFormatSizeLicensePublisher/Copright statementContent
acs.jpclett.1c04011.pdftextAdobe PDF1.53 MBpublishedOpen
BORIS Portal
Bern Open Repository and Information System
Build: d1c7f7 [27.06. 13:56]
Explore
  • Projects
  • Funding
  • Publications
  • Research Data
  • Organizations
  • Researchers
More
  • About BORIS Portal
  • Send Feedback
  • Cookie settings
  • Service Policy
Follow us on
  • Mastodon
  • YouTube
  • LinkedIn
UniBe logo