Biocatalytic tools for enzymatic cascades

The field of biocatalysis is receiving increased attention over the years, owing to the incontrovertible necessity for sustainable processes. As essential part of biocatalysis, enzymes fulfil the demand for greener reactions thanks to their valuable properties, while providing simpler synthetic routes with higher selectivity than the traditional hazardous methods. Potentially, enzymes are remarkable catalysts but most of the times, they require special optimization before being effectively applied at industrial scale, which translates into significant investment of work and time. In this regard, the combination of enzymes as tool to assist a reaction or to carry out particular functions can facilitate the process.
A series of biocatalytic tools have been created in this work to tackle specific problems. Afterwards, they were tested to evaluate their efficacy during the utilization.

2022

dissertation

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