Characterization of the tobacco eIF-4A gene family

BORIS DOI
Publisher DOI
Description
Characterization of cDNAs encoding eukaryotic translation initiation factor 4A (eIF-4A) indicates the expression of a minimum of ten related genes in tobacco leaf cells. The ten groups fall into two gene families, NeIF-4A2 and NeIF-4A3. The majority of the cDNAs exhibit significant sequence similarity to the NeIF-4A2 family at both the DNA and deduced amino acid levels. Northern analysis using specific probes indicates variable expression of four family members in various tobacco organs. Western analysis, using an anti-tobacco eIF-4A polyclonal antibody, reveals a complex pattern of immunologically related polypeptides of approximately 46 kDa. Subcellular fractionation suggests that at least one eIF-4A-related polypeptide is located in the chloroplast where it is ribosome-associated.
Date of Publication
1994-12
Publication Type
Article
Subject(s)
Keyword(s)
cDNA gene family
•
NeIF-4A
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RNA helicase
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translation initiation factor
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Nicotiana
Language(s)
en
Contributor(s)
Owttrim, George W
Thomas, Adri AM
Additional Credits
Series
Plant molecular biology
Publisher
Springer
ISSN
0167-4412
Access(Rights)
open.access
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