Macrophage phagocytosis of SARS-CoV-2-infected cells mediates potent plasmacytoid dendritic cell activation.
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BORIS DOI
Date of Publication
July 2023
Publication Type
Article
Division/Institute
Author
Subject(s)
Series
Cellular & molecular immunology
ISSN or ISBN (if monograph)
1672-7681
Publisher
Nature Publ. Group
Language
English
Publisher DOI
PubMed ID
37253946
Uncontrolled Keywords
Description
Early and strong interferon type I (IFN-I) responses are usually associated with mild COVID-19 disease, whereas persistent or unregulated proinflammatory cytokine responses are associated with severe disease outcomes. Previous work suggested that monocyte-derived macrophages (MDMs) are resistant and unresponsive to SARS-CoV-2 infection. Here, we demonstrate that upon phagocytosis of SARS-CoV-2-infected cells, MDMs are activated and secrete IL-6 and TNF. Importantly, activated MDMs in turn mediate strong activation of plasmacytoid dendritic cells (pDCs), leading to the secretion of high levels of IFN-α and TNF. Furthermore, pDC activation promoted IL-6 production by MDMs. This kind of pDC activation was dependent on direct integrin-mediated cell‒cell contacts and involved stimulation of the TLR7 and STING signaling pathways. Overall, the present study describes a novel and potent pathway of pDC activation that is linked to the macrophage-mediated clearance of infected cells. These findings suggest that a high infection rate by SARS-CoV-2 may lead to exaggerated cytokine responses, which may contribute to tissue damage and severe disease.
File(s)
File | File Type | Format | Size | License | Publisher/Copright statement | Content | |
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s41423-023-01039-4.pdf | text | Adobe PDF | 2.16 MB | Attribution (CC BY 4.0) | published |