Publication:
Marasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect

cris.virtualsource.author-orcidcb6eaa41-620d-434d-b861-2d142a87a6df
cris.virtualsource.author-orcidfa117f7e-3334-4dad-bf3b-8b5c682640f7
cris.virtualsource.author-orcidcb3c94c2-7095-4ade-8db1-ee321fde55f9
cris.virtualsource.author-orcid334dfc07-e0e2-472b-8a7f-6d2550d75859
cris.virtualsource.author-orcidf270f624-7653-42e1-b355-6d20904dee07
datacite.rightsopen.access
dc.contributor.authorMoradi, Aboubakr
dc.contributor.authorAusterlitz, Tina
dc.contributor.authorDahlin, Paul
dc.contributor.authorRobert, Christelle Aurélie Maud
dc.contributor.authorMaurer, Corina
dc.contributor.authorSteinauer, Katja
dc.contributor.authorDoan, Van Cong
dc.contributor.authorHimmighofen, Paul Anton
dc.contributor.authorWieczorek, Krzysztof
dc.contributor.authorKuenzler, Markus
dc.contributor.authorMauch, Felix
dc.date.accessioned2024-10-05T12:24:06Z
dc.date.available2024-10-05T12:24:06Z
dc.date.issued2021-09-01
dc.description.abstractBackground Plant-parasitic nematodes and herbivorous insects have a significant negative impact on global crop production. A successful approach to protect crops from these pests is the in planta expression of nematotoxic or entomotoxic proteins such as crystal proteins from Bacillus thuringiensis (Bt) or plant lectins. However, the efficacy of this approach is threatened by emergence of resistance in nematode and insect populations to these proteins. To solve this problem, novel nematotoxic and entomotoxic proteins are needed. During the last two decades, several cytoplasmic lectins from mushrooms with nematicidal and insecticidal activity have been characterized. In this study, we tested the potential of Marasmius oreades agglutinin (MOA) to furnish Arabidopsis plants with resistance towards three economically important crop pests: the two plant-parasitic nematodes Heterodera schachtii and Meloidogyne incognita and the herbivorous diamondback moth Plutella xylostella. Results The expression of MOA does not affect plant growth under axenic conditions which is an essential parameter in the engineering of genetically modified crops. The transgenic Arabidopsis lines showed nearly complete resistance to H. schachtii, in that the number of female and male nematodes per cm root was reduced by 86-91 % and 43-93 % compared to WT, respectively. M. incognita proved to be less susceptible to the MOA protein in that 18-25 % and 26-35 % less galls and nematode egg masses, respectively, were observed in the transgenic lines. Larvae of the herbivorous P. xylostella foraging on MOA-expression lines showed a lower relative mass gain (22-38 %) and survival rate (15-24 %) than those feeding on WT plants. Conclusions The results of our in planta experiments reveal a robust nematicidal and insecticidal activity of the fungal lectin MOA against important agricultural pests which may be exploited for crop protection.
dc.description.sponsorshipInstitut für Pflanzenwissenschaften (IPS)
dc.identifier.doi10.48350/159676
dc.identifier.pmid34470613
dc.identifier.publisherDOI10.1186/s12870-021-03186-0
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/57280
dc.language.isoen
dc.publisherBioMed Central
dc.relation.ispartofBMC plant biology
dc.relation.issn1471-2229
dc.relation.organizationDCD5A442C222E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442C579E17DE0405C82790C4DE2
dc.subjectMarasmius oreades agglutinin
dc.subjectArabidopsis
dc.subjectHeterodera schachtii
dc.subjectMeloidogyne incognita
dc.subjectPlutella xylostella
dc.subject.ddc500 - Science::580 - Plants (Botany)
dc.titleMarasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect
dc.typearticle
dspace.entity.typePublication
dspace.file.typetext
oaire.citation.issue1
oaire.citation.startPage402
oaire.citation.volume21
oairecerif.author.affiliationInstitut für Pflanzenwissenschaften (IPS)
oairecerif.author.affiliationInstitut für Pflanzenwissenschaften (IPS)
oairecerif.author.affiliationInstitut für Pflanzenwissenschaften (IPS)
oairecerif.author.affiliationInstitut für Pflanzenwissenschaften (IPS)
oairecerif.author.affiliationInstitut für Pflanzenwissenschaften (IPS)
oairecerif.identifier.urlhttps://rdcu.be/cyByu
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unibe.date.licenseChanged2021-10-13 08:32:23
unibe.description.ispublishedpub
unibe.eprints.legacyId159676
unibe.journal.abbrevTitleBMC PLANT BIOL
unibe.refereedtrue
unibe.subtype.articlejournal

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