Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia
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BORIS DOI
Date of Publication
2014
Publication Type
Article
Division/Institute
Author
Calandriello, Elena | |
Wuertz-Kozak, Karin | |
Keel, Marius JB | |
Chan, Samantha CW |
Series
BMC musculoskeletal disorders
ISSN or ISBN (if monograph)
1471-2474
Publisher
BioMed Central
Language
English
Publisher DOI
PubMed ID
25496082
Description
Notochordal cells (NC) remain in the focus of research for regenerative therapy for the degenerated intervertebral disc (IVD) due to their progenitor status. Recent findings suggested their regenerative action on more mature disc cells, presumably by the secretion of specific factors, which has been described as notochordal cell conditioned medium (NCCM). The aim of this study was to determine NC culture conditions (2D/3D, fetal calf serum, oxygen level) that lead to significant IVD cell activation in an indirect co-culture system under normoxia and hypoxia (2% oxygen).