Publication:
Role of KCNMA1 in breast cancer

cris.virtual.author-orcid0000-0001-6741-3000
cris.virtualsource.author-orcid6c84719c-dd7a-4678-aa8a-1b4f99d3856a
dc.contributor.authorOeggerli, Martin
dc.contributor.authorTian, Yuemin
dc.contributor.authorRuiz, Christian
dc.contributor.authorWijker, Barbara
dc.contributor.authorSauter, Guido
dc.contributor.authorObermann, Ellen
dc.contributor.authorGüth, Uwe
dc.contributor.authorZlobec, Inti
dc.contributor.authorSausbier, Matthias
dc.contributor.authorKunzelmann, Karl
dc.contributor.authorBubendorf, Lukas
dc.date.accessioned2024-10-11T13:16:20Z
dc.date.available2024-10-11T13:16:20Z
dc.date.issued2012
dc.description.abstractKCNMA1 encodes the α-subunit of the large conductance, voltage and Ca(2+)-activated (BK) potassium channel and has been reported as a target gene of genomic amplification at 10q22 in prostate cancer. To investigate the prevalence of the amplification in other human cancers, the copy number of KCNMA1 was analyzed by fluorescence-in-situ-hybridization (FISH) in 2,445 tumors across 118 different tumor types. Amplification of KCNMA1 was restricted to a small but distinct fraction of breast, ovarian and endometrial cancer with the highest prevalence in invasive ductal breast cancers and serous carcinoma of ovary and endometrium (3-7%). We performed an extensive analysis on breast cancer tissue microarrays (TMA) of 1,200 tumors linked to prognosis. KCNMA1 amplification was significantly associated with high tumor stage, high grade, high tumor cell proliferation, and poor prognosis. Immunofluorescence revealed moderate or strong KCNMA1 protein expression in 8 out of 9 human breast cancers and in the breast cancer cell line MFM223. KCNMA1-function in breast cancer cell lines was confirmed by whole-cell patch clamp recordings and proliferation assays, using siRNA-knockdown, BK channel activators such as 17ß-estradiol and the BK-channel blocker paxilline. Our findings revealed that enhanced expression of KCNMA1 correlates with and contributes to high proliferation rate and malignancy of breast cancer.
dc.description.numberOfPages1
dc.description.sponsorshipInstitut für Pathologie
dc.identifier.doi10.7892/boris.12498
dc.identifier.isi000307380900014
dc.identifier.pmid22899999
dc.identifier.publisherDOI10.1371/journal.pone.0041664
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/82593
dc.language.isoen
dc.publisherPublic Library of Science
dc.publisher.placeLawrence, Kans.
dc.relation.ispartofPLoS ONE
dc.relation.issn1932-6203
dc.relation.organizationDCD5A442BF89E17DE0405C82790C4DE2
dc.titleRole of KCNMA1 in breast cancer
dc.typearticle
dspace.entity.typePublication
dspace.file.typetext
oaire.citation.issue8
oaire.citation.startPagee41664
oaire.citation.volume7
oairecerif.author.affiliationInstitut für Pathologie
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unibe.description.ispublishedpub
unibe.eprints.legacyId12498
unibe.journal.abbrevTitlePLOS ONE
unibe.subtype.articlejournal

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