Thyroid signaling in immune organs and cells of the teleost fish rainbow trout (Oncorhynchus mykiss).
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BORIS DOI
Date of Publication
May 2014
Publication Type
Article
Division/Institute
Subject(s)
Series
Fish & shellfish immunology
ISSN or ISBN (if monograph)
1050-4648
Publisher
Elsevier Science
Language
English
Publisher DOI
PubMed ID
24657316
Uncontrolled Keywords
Description
Thyroid hormones are involved in modulating the immune system in mammals. In contrast, there is no information on the role played by these hormones in the immune system of teleost fish. Here we provide initial evidence for the presence of active thyroid signaling in immune organs and cells of teleosts. We demonstrate that immune organs (head kidney and spleen) and isolated leukocytes (from head kidney and peripheral blood) of the rainbow trout (Oncorhynchus mykiss) express both thyroid receptor α (THRA) and β (THRB). Absolute mRNA levels of THRA were significantly higher than those of THRB. THRA showed higher expression in immune organs and isolated immune cells compared to the reference organ, liver, while THRB showed the opposite. In vivo exposure of trout to triiodothryronine (T3) or the anti-thyroid agent propylthiouracil (PTU) altered THR expression in immune organs and cells. Effect of T3 and PTU over the relative expression of selected marker genes of immune cell subpopulations was also studied. Treatments changed the relative expression of markers of cytotoxic, helper and total T cells (cd4, cd8a, trb), B lymphocytes (mIgM) and macrophages (csf1r). These findings suggest that the immune system of rainbow trout is responsive to thyroid hormones.
File(s)
File | File Type | Format | Size | License | Publisher/Copright statement | Content | |
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1-s2.0-S1050464814000801-main.pdf | text | Adobe PDF | 857.33 KB | publisher | published |