Time Dependence of Elimination of Different PEth Homologues in Alcoholics in Comparison with Social Drinkers
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BORIS DOI
Publisher DOI
PubMed ID
24471840
Description
BACKGROUND: Phosphatidylethanol (PEth) is a direct marker of alcohol consumption, which has been known for almost 30 years. Each PEth molecule carries 2 fatty acids, which differ in chain length and degree of unsaturation. It is formed by means of phospholipase D in the presence of ethanol. Usually, this marker was used by quantification of the PEth homologue 16:0/18:1. The intention of this work was to get more information about the distribution and the quantity of the different PEth homologues.
METHODS: Blood samples from 12 alcohol-dependent subjects were collected and analyzed during withdrawal therapy. For comparison, blood from 78 healthy social drinkers was also analyzed. PEth analysis was performed as follows: after liquid-liquid extraction, the homologues were separated on a Luna Phenyl Hexyl column, injected to an HPLC system (1100 system; Agilent) and identified by ESI-MS/MS (QTrap 2000; AB Sciex) using multiple reaction monitoring.
RESULTS: PEth 16:0/18:1 is the major homologue comparing the area ratios of PEth homologues in blood samples from alcoholics. Additional prevalent homologues were PEth 16:0/18:2, 18:0/18:2, and 18:0/18:1. The homologues occurring in blood samples from alcoholics as well as from social drinkers were mostly the same, but differences among their distribution pattern were observed.
CONCLUSIONS: In addition to the approach to quantitate the PEth homologue
16:0/18:1, this is a new and alternative proceeding for the differentiation
between alcoholics and social drinkers using this alcohol consumption marker.
METHODS: Blood samples from 12 alcohol-dependent subjects were collected and analyzed during withdrawal therapy. For comparison, blood from 78 healthy social drinkers was also analyzed. PEth analysis was performed as follows: after liquid-liquid extraction, the homologues were separated on a Luna Phenyl Hexyl column, injected to an HPLC system (1100 system; Agilent) and identified by ESI-MS/MS (QTrap 2000; AB Sciex) using multiple reaction monitoring.
RESULTS: PEth 16:0/18:1 is the major homologue comparing the area ratios of PEth homologues in blood samples from alcoholics. Additional prevalent homologues were PEth 16:0/18:2, 18:0/18:2, and 18:0/18:1. The homologues occurring in blood samples from alcoholics as well as from social drinkers were mostly the same, but differences among their distribution pattern were observed.
CONCLUSIONS: In addition to the approach to quantitate the PEth homologue
16:0/18:1, this is a new and alternative proceeding for the differentiation
between alcoholics and social drinkers using this alcohol consumption marker.
Date of Publication
2014-02
Publication Type
Article
Subject(s)
Language(s)
en
Contributor(s)
Gnann, Heike | |
Thierauf, Annette | |
Hagenbuch, Friedemann | |
Röhr, Bernhard |
Additional Credits
Series
Alcoholism: clinical and experimental research
Publisher
Wiley-Blackwell
ISSN
0145-6008
Access(Rights)
restricted