Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei
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BORIS DOI
Date of Publication
December 15, 2017
Publication Type
Article
Division/Institute
Author
Schmidt, Remo S. | |
Kunz Renggli, Christina | |
Mäser, Pascal |
Series
PLoS ONE
ISSN or ISBN (if monograph)
1932-6203
Publisher
Public Library of Science
Language
English
Publisher DOI
PubMed ID
29244877
Description
CLC type anion transport proteins are homo-dimeric or hetero-dimeric with an integrated transport function in each subunit. We have identified and partially characterized three members of this family named TbVCL1, TbVCL2 and TbVCL3 in Trypanosoma brucei. Among the human CLC family members, the T. brucei proteins display highest similarity to CLC-6 and CLC-7. TbVCL1, but not TbVCL2 and TbVCL3 is able to complement growth of a CLC-deficient Saccharomyces cerevisiae mutant. All TbVCL-HA fusion proteins localize intracellulary in procyclic form trypanosomes. TbVCL1 localizes close to the Golgi apparatus and TbVCL2 and TbVCL3 to the endoplasmic reticulum. Upon expression in Xenopus oocytes, all three proteins induce similar outward rectifying chloride ion currents. Currents are sensitive to low concentrations of DIDS, insensitive to the pH in the range 5.4 to 8.4 and larger in nitrate than in chloride medium.
File(s)
File | File Type | Format | Size | License | Publisher/Copright statement | Content | |
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2017_PLOSOne_12_e0188219.pdf | text | Adobe PDF | 7.37 MB | Attribution (CC BY 4.0) | published |