Compression and crevasses in vitreous sections under different cutting conditions.
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BORIS DOI
Date of Publication
May 2008
Publication Type
Article
Division/Institute
Author
Han, H-M | |
Dubochet, J |
Subject(s)
Series
Journal of microscopy
ISSN or ISBN (if monograph)
0022-2720
Publisher
Wiley-Blackwell
Language
English
Publisher DOI
PubMed ID
18445144
Description
Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.
File(s)
| File | File Type | Format | Size | License | Publisher/Copright statement | Content | |
|---|---|---|---|---|---|---|---|
| j.1365-2818.2008.01972.x.pdf | text | Adobe PDF | 312.53 KB | publisher | published |