Publication:
In Vitro Effect of Combined Hypertonic Saline and Salbutamol on Ciliary Beating Frequency and Mucociliary Transport in Human Nasal Epithelial Cells of Healthy Volunteers and Patients with Cystic Fibrosis.

cris.virtual.author-orcid0000-0003-1741-5221
cris.virtual.author-orcid0000-0003-3880-4437
cris.virtual.author-orcid0000-0002-6741-9434
cris.virtual.author-orcid0000-0002-5239-1571
cris.virtual.author-orcid0000-0003-4754-1608
cris.virtualsource.author-orcid6d13ac32-31b5-4f74-9c32-27319aad6375
cris.virtualsource.author-orcide3d61a65-3b66-4cf4-a428-a08ddc854d50
cris.virtualsource.author-orcid0b71a1e3-5226-44b7-a1cb-aa7409b1b322
cris.virtualsource.author-orcid4006c15a-163a-4cd7-8f9d-7de5aff0e9fa
cris.virtualsource.author-orcidaeba9e0a-b3a9-4d7a-8207-e34ae1262581
cris.virtualsource.author-orcid2fc4503c-3aa6-4936-8ab7-fd74929a8b74
cris.virtualsource.author-orcid7457a40a-9226-489a-9650-a936c14fb53f
cris.virtualsource.author-orcide2cc911c-ee16-4177-9c75-648bb31ce324
cris.virtualsource.author-orcidb7d49b7e-18d4-45bc-99e8-c1c42ce113e5
datacite.rightsrestricted
dc.contributor.authorEscher, Anaïs Estelle
dc.contributor.authorKieninger, Elisabeth
dc.contributor.authorDe Groof, Susan
dc.contributor.authorSavas, Sibel T
dc.contributor.authorSchneiter, Martin Michael
dc.contributor.authorTschanz, Stefan A.
dc.contributor.authorFrenz, Martin
dc.contributor.authorLatzin, Philipp
dc.contributor.authorCasaulta, Carmen
dc.contributor.authorMüller, Loretta Lina
dc.date.accessioned2024-10-25T16:31:22Z
dc.date.available2024-10-25T16:31:22Z
dc.date.issued2023-08
dc.description.abstractBackground: Inhalation of hypertonic saline (HS) is standard of care in patients with cystic fibrosis (CF). However, it is unclear if adding salbutamol has-besides bronchodilation-further benefits, for example, on the mucociliary clearance. We assessed this in vitro by measuring the ciliary beating frequency (CBF) and the mucociliary transport rate (MCT) in nasal epithelial cells (NECs) of healthy volunteers and patients with CF. Aims: To investigate the effect of HS, salbutamol, and its combination on (muco)ciliary activity of NECs in vitro, and to assess potential differences between healthy controls and patients with CF. Methods: NECs obtained from 10 healthy volunteers and 5 patients with CF were differentiated at the air-liquid interface and aerosolized with 0.9% isotonic saline ([IS] control), 6% HS, 0.06% salbutamol, or combined HS and salbutamol. CBF and MCT were monitored over 48-72 hours. Results: In NECs of healthy controls, the absolute CBF increase was comparable for all substances, but CBF dynamics were different: HS increased CBF slowly and its effect lasted for an extended period, salbutamol and IS increased CBF rapidly and the effect subsided similarly fast, and HS and salbutamol resulted in a rapid and long-lasting CBF increase. Results for CF cells were comparable, but less pronounced. Similar to CBF, MCT increased after the application of all the tested substances. Conclusion: CBF and MCT of NECs of healthy participants and CBF of patients with CF increased upon treatment with aerosolized IS, HS, salbutamol, or HS and salbutamol, showing a relevant effect for all tested substances. The difference in the CBF dynamics can be explained by the fact that the properties of the mucus are changed differently by different saline concentrations.
dc.description.numberOfPages10
dc.description.sponsorshipInstitut für Angewandte Physik (IAP)
dc.description.sponsorshipUniversitätsklinik für Kinderheilkunde - Pneumologie / Allergologie
dc.description.sponsorshipUniversitätsklinik für Kinderheilkunde
dc.description.sponsorshipDepartment for BioMedical Research, Forschungsgruppe Pneumologie (Pädiatrie)
dc.description.sponsorshipInstitut für Anatomie - Rotation Anatomie
dc.description.sponsorshipInstitut für Anatomie
dc.identifier.doi10.48350/182664
dc.identifier.pmid37196208
dc.identifier.publisherDOI10.1089/jamp.2022.0026
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/167211
dc.language.isoen
dc.publisherMary Ann Liebert
dc.relation.ispartofJournal of aerosol medicine and pulmonary drug delivery
dc.relation.issn1941-2711
dc.relation.organizationDCD5A442BADAE17DE0405C82790C4DE2
dc.relation.organizationDCD5A442BCD7E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442BD18E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442BED5E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442C068E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442C249E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442C2BAE17DE0405C82790C4DE2
dc.subjectair–liquid interface cell cultures ciliary motility cystic fibrosis inhalation primary ciliary dyskinesia
dc.subject.ddc600 - Technology::610 - Medicine & health
dc.subject.ddc500 - Science::570 - Life sciences; biology
dc.subject.ddc600 - Technology::620 - Engineering
dc.titleIn Vitro Effect of Combined Hypertonic Saline and Salbutamol on Ciliary Beating Frequency and Mucociliary Transport in Human Nasal Epithelial Cells of Healthy Volunteers and Patients with Cystic Fibrosis.
dc.typearticle
dspace.entity.typePublication
dspace.file.typetext
oaire.citation.endPage180
oaire.citation.issue4
oaire.citation.startPage171
oaire.citation.volume36
oairecerif.author.affiliationUniversitätsklinik für Kinderheilkunde
oairecerif.author.affiliationUniversitätsklinik für Kinderheilkunde
oairecerif.author.affiliationUniversitätsklinik für Kinderheilkunde
oairecerif.author.affiliationInstitut für Anatomie - Rotation Anatomie
oairecerif.author.affiliationInstitut für Anatomie
oairecerif.author.affiliationInstitut für Angewandte Physik (IAP)
oairecerif.author.affiliationUniversitätsklinik für Kinderheilkunde - Pneumologie / Allergologie
oairecerif.author.affiliationUniversitätsklinik für Kinderheilkunde
oairecerif.author.affiliationDepartment for BioMedical Research, Forschungsgruppe Pneumologie (Pädiatrie)
oairecerif.author.affiliation2Department for BioMedical Research (DBMR)
oairecerif.author.affiliation2Institut für Anatomie
oairecerif.author.affiliation2Institut für Anatomie - PCD-UNIBE Stereologie & Zentrale Dienste
oairecerif.author.affiliation2Institut für Angewandte Physik (IAP) - Biomedizinische Photonik
oairecerif.author.affiliation2Universitätsklinik für Kinderheilkunde
oairecerif.author.affiliation2Universitätsklinik für Kinderheilkunde
oairecerif.author.affiliation3Institut für Angewandte Physik (IAP) - Biomedizinische Photonik
oairecerif.author.affiliation3Department for BioMedical Research, Forschungsgruppe Pneumologie (Pädiatrie)
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unibe.date.licenseChanged2023-05-19 09:53:34
unibe.description.ispublishedpub
unibe.eprints.legacyId182664
unibe.journal.abbrevTitleJ AEROSOL MED PULM D
unibe.refereedTRUE
unibe.subtype.articlejournal

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