Establishment of a protocol for preventive vaccination against equine insect bite hypersensitivity.
Publisher DOI
PubMed ID
36272211
Abstract
Insect bite hypersensitivity (IBH) is a seasonal dermatitis of horses caused by IgE-mediated reactions to bites of Culicoides midges characterized by an imbalance of T-cell subsets. Iceland is free of the causative species but the prevalence of IBH in exported Icelandic horses is especially high. We have shown that intralymphatic (i.l.) vaccination with r-Culicoides allergens in Aluminum hydroxide (alum) and monophosphoryl lipid A (MPLA) adjuvants induces a desired Th1/regulatory T-cell response. The aim of this study was to compare i.l. to subcutaneous (s.c.) injection. Twelve healthy Icelandic horses were injected, i.l. or s.c., three times with four-week interval, using purified r-Culicoides allergens in alum/MPLA. Serum antibody levels and cytokine profile following in vitro re-stimulation of PBMC were analysed. Comparable allergen-specific IgG antibodies were induced following both routes of vaccinations. The antibodies showed similar capacity to block binding of IgE from IBH-affected horse to the allergens. Upon re-stimulation of PBMC, IL-10 was induced. Horses vaccinated i.l. produced more IFN-γ and less IL-4 as compared to the horses injected s.c., but the difference did not reach significance. It can be concluded that applying the simpler s.c. injection instead of i.l. to obtain a suitable immune response could be option in IBH immunotherapy.
Date Issued
2022-11
Publication Type
Article
Subjects
Aluminum hydroxide
•
Insect bite hypersensitivity
•
Intralymphatic injection
•
Monophosphoryl lipid A
•
Preventive allergen immunotherapy
•
Subcutaneous injection
Language(s)
en
Author(s)
Stefansdottir, Sara Bjork | |
Jonsdottir, Sigridur | |
Kristjansdottir, Holmfridur | |
Svansson, Vilhjalmur | |
Torsteinsdottir, Sigurbjorg |
Journal
Veterinary Immunology and Immunopathology
Publisher
Elsevier
ISSN
0165-2427
Access(Rights)
open.access