Preparation of soluble extracts from adenovirus-infected cells for studies of RNA splicing
Abstract
Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro using T7 RNA polymerase-derived splicing substrate RNAs.
Date Issued
2007
Publication Type
Article
Language(s)
en
Author(s)
Akusjarvi, Goran |
Additional Credits
Journal
Methods in molecular medicine
Publisher
Humana Press
ISSN
1543-1894
Access(Rights)
metadata.only