Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing
Publisher DOI
PubMed ID
24679958
Abstract
Diagnostic tests based on cell-mediated immunity are used in programmes for eradication of bovine tuberculosis (Mycobacterium bovis). Serological assays could be applied as ancillary methods to detect infected animals. Our objective was to evaluate two serological techniques: M. bovis Ab Test (IDEXX, USA) and Enferplex™ TB assay (Enfer, Ireland) in animals tested simultaneously with the single and comparative intradermal tests and the interferon-gamma assay. This work was performed at two stages. First, a preliminary panel of samples collected prior to intradermal tests from tuberculosis-free (n=60) and M. bovis-infected herds (n=78) was assayed, obtaining high specificity: 100% (M. bovis Ab Test) and 98.3% (Enferplex TB assay) but low sensitivity (detection of M. bovis infected animals): 23.9% (M. bovis Ab Test) and 32.6% (Enferplex TB assay). Subsequently, the use of serological techniques was further studied in two herds with M. bovis infection (n=77) using samples collected prior to, and 72 h and 15 days after PPD inoculation. The highest level of detection of infected animals for serology was achieved at 15 days post-intradermal tests taking advantage of the anamnestic effect: 70.4% and 85.2% in herd A, and 66.7% and 83.3% in herd B, using M. bovis Ab Test and Enferplex TB assay, respectively. Quantitative results (average values obtained with M. bovis Ab Test ELISA and degree of positivity obtained with Enferplex TB assay) were higher in animals showing lesions compatible with tuberculosis. No significant differences were observed in the number of confirmed infected animals detected with either serological technique.
Date Issued
2014-06-04
Publication Type
Article
Subject(s)
Subjects
Bovine tuberculosis
•
Diagnostic tests
•
Serological response
•
Boosting effect
Language(s)
en
Author(s)
Casal, Carmen | |
Díez-Guerrier, Alberto | |
Álvarez, Julio | |
Mateos, Ana | |
Linscott, Richard | |
Martel, Edmond | |
Lawrence, John C | |
Whelan, Clare | |
Clarke, John | |
O'Brien, Amanda | |
Domínguez, Lucas | |
Aranaz, Alicia |
Additional Credits
Journal
Veterinary microbiology
Publisher
Elsevier
ISSN
0378-1135
Access(Rights)
restricted