Conditioned Medium of Demineralized Freeze-Dried Bone Activates Gene Expression in Periodontal Fibroblasts in Vitro.
Publisher DOI
PubMed ID
25786563
Abstract
BACKGROUND:
Demineralized bone matrix (DBM) is used for the treatment of osseous defects. Conditioned medium from native bone chips can activate transforming growth factor (TGF)-β signaling in mesenchymal cells. The aim of the study was to determine whether processing of native bone into DBM affects the activity of the conditioned medium.
METHODS:
Porcine cortical bone blocks were subjected to defatting, different concentrations of hydrochloric acid and various temperatures. DBM was lyophilized, ground, and placed into culture medium. Human gingiva and periodontal fibroblasts were exposed to the respective conditioned medium (DBCM). Changes in the expression of TGF-β target genes were determined.
RESULTS:
DBCM altered the expression of TGF-β target genes, e.g., adrenomedullin, pentraxin 3, KN Motif And Ankyrin Repeat Domains 4, interleukin 11, NADPH oxidase 4, and BTB (POZ) Domain Containing 11, by at least five-fold. The response was observed in fibroblasts from both sources. Defatting lowered the activity of DBCM. The TGF-β receptor type I kinase inhibitor SB431542, but not the inhibitor of bone morphogenetic protein receptor dorsomorphin, blocked the effects of DBCM on gene expression. Moreover, conditioned medium obtained from commercial human DBM modulated the expression of TGF-β target genes.
CONCLUSION:
The findings suggest that the conditioned medium from demineralized bone matrix can activate TGF-β signaling in oral fibroblasts.
KEYWORDS:
TGF-beta superfamily proteins; bone; bone substitutes; bone transplantation; conditioned media; freeze drying
Demineralized bone matrix (DBM) is used for the treatment of osseous defects. Conditioned medium from native bone chips can activate transforming growth factor (TGF)-β signaling in mesenchymal cells. The aim of the study was to determine whether processing of native bone into DBM affects the activity of the conditioned medium.
METHODS:
Porcine cortical bone blocks were subjected to defatting, different concentrations of hydrochloric acid and various temperatures. DBM was lyophilized, ground, and placed into culture medium. Human gingiva and periodontal fibroblasts were exposed to the respective conditioned medium (DBCM). Changes in the expression of TGF-β target genes were determined.
RESULTS:
DBCM altered the expression of TGF-β target genes, e.g., adrenomedullin, pentraxin 3, KN Motif And Ankyrin Repeat Domains 4, interleukin 11, NADPH oxidase 4, and BTB (POZ) Domain Containing 11, by at least five-fold. The response was observed in fibroblasts from both sources. Defatting lowered the activity of DBCM. The TGF-β receptor type I kinase inhibitor SB431542, but not the inhibitor of bone morphogenetic protein receptor dorsomorphin, blocked the effects of DBCM on gene expression. Moreover, conditioned medium obtained from commercial human DBM modulated the expression of TGF-β target genes.
CONCLUSION:
The findings suggest that the conditioned medium from demineralized bone matrix can activate TGF-β signaling in oral fibroblasts.
KEYWORDS:
TGF-beta superfamily proteins; bone; bone substitutes; bone transplantation; conditioned media; freeze drying
Date Issued
2015-06
Publication Type
Article
Subject(s)
Subjects
Bone and bones
•
TGF-beta superfamily proteins
•
bone substitutes
•
bone transplantation
•
culture media - conditioned
•
freeze drying
Language(s)
en
Author(s)
Aurélio Bianchini, M |
Journal
Journal of periodontology
Publisher
American Academy of Periodontology
ISSN
0022-3492
Access(Rights)
restricted