Epidemiological, serological, and viral genomic analysis of an outbreak of elephant hemorrhagic disease in Switzerland.

Abstract

Elephant hemorrhagic disease (EHD), caused by several Elephant endotheliotropic herpesviruses (EEHV), represents a frequently lethal syndrome, affecting both captive and free-living elephants. In the summer of 2022, three young Asian elephants (Elephas maximus) succumbed to EHD in a zoo in Switzerland, despite considerable preventive efforts and early detection of EEHV1A viremia. In this communication, we describe the extent of preventive measures in terms of prior virus detection, active survey of viremia, and antibody status. The results show that: (1) A previously undetected EEHV1A strain had remained unrecognized among these elephants. Probably, the virus re-emerged after almost 40 years of latency from one of the oldest elephants in the zoo. (2) While two of the three affected animals had prior immune responses against EEHV1, their strain-specific immunity proved insufficient to prevent EHD. The complete genomic DNA sequence of the EEHV1A strain involved was determined, and detailed comparisons with multiple EEHV1 strains were made, revealing a much greater extent of divergence and level of complexity among the encoded proteins than previously described. Overall, these data confirmed that all three EHD cases here had been infected by the same novel strain of EEHV subtype 1A.