Development of an array of ion-selective microelectrodes aimed for the monitoring of extracellular ionic activities
Publisher DOI
PubMed ID
17073412
Abstract
In this study, we present the development and the characterization of a generic platform for cell culture able to monitor extracellular ionic activities (K+, NH4+) for real-time monitoring of cell-based responses, such as necrosis, apoptosis, or differentiation. The platform for cell culture is equipped with an array of 16 silicon nitride micropipet-based ion-selective microelectrodes with a diameter of either 2 or 6 microm. This array is located at the bottom of a 200-microm-wide and 350-microm-deep microwell where the cells are cultured. The characterization of the ion-selective microelectrode arrays in different standard and physiological solutions is presented. Near-Nernstian slopes were obtained for potassium- (58.6 +/- 0.8 mV/pK, n = 15) and ammonium-selective microelectrodes (59.4 +/- 3.9 mV/pNH4, n = 13). The calibration curves were highly reproducible and showed an average drift of 4.4 +/- 2.3 mV/h (n = 10). Long-term behavior and response after immersion in physiological solutions are also presented. The lifetime of the sensors was found to be extremely long with a high recovery rate.
Date Issued
2006
Publication Type
Article
Language(s)
en
Author(s)
Generelli, Silvia | |
de Rooij, Nicolaas F | |
Koudelka-Hep, Milena | |
Berthiaume, François | |
Yarmush, Martin L |
Additional Credits
Journal
Analytical chemistry
Publisher
American Chemical Society
ISSN
0003-2700
Access(Rights)
metadata.only