An Innate Checkpoint Determines Immune Dysregulation and Immunopathology during Pulmonary Murine Coronavirus Infection.
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BORIS DOI
Date of Publication
March 15, 2023
Publication Type
Article
Division/Institute
Author
Grabherr, Sarah | |
Waltenspühl, Alexandra | |
Büchler, Lorina | |
Lütge, Mechthild | |
Cheng, Hung-Wei | |
Caviezel-Firner, Sonja | |
Ludewig, Burkhard | |
Pikor, Natalia B |
Series
Journal of immunology
ISSN or ISBN (if monograph)
1550-6606
Publisher
American Association of Immunologists
Language
English
Publisher DOI
PubMed ID
36715496
Description
Hallmarks of life-threatening, coronavirus-induced disease include dysregulated antiviral immunity and immunopathological tissue injury. Nevertheless, the sampling of symptomatic patients overlooks the initial inflammatory sequela culminating in severe coronavirus-induced disease, leaving a fundamental gap in our understanding of the early mechanisms regulating anticoronavirus immunity and preservation of tissue integrity. In this study, we delineate the innate regulators controlling pulmonary infection using a natural mouse coronavirus. Within hours of infection, the cellular landscape of the lung was transcriptionally remodeled altering host metabolism, protein synthesis, and macrophage maturation. Genetic perturbation revealed that these transcriptional programs were type I IFN dependent and critically controlled both host cell survival and viral spread. Unrestricted viral replication overshooting protective IFN responses culminated in increased IL-1β and alarmin production and triggered compensatory neutrophilia, interstitial inflammation, and vascular injury. Thus, type I IFNs critically regulate early viral burden, which serves as an innate checkpoint determining the trajectory of coronavirus dissemination and immunopathology.
File(s)
File | File Type | Format | Size | License | Publisher/Copright statement | Content | |
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ji2200533.pdf | text | Adobe PDF | 4.44 MB | publisher | published |