Publication:
Replication and single-cycle delivery of SARS-CoV-2 replicons.

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cris.virtual.author-orcid0000-0003-3906-4629
cris.virtual.author-orcid0000-0001-8554-790X
cris.virtualsource.author-orcid9aa7a89d-109a-482e-80f3-8c6ed7312227
cris.virtualsource.author-orcidece3e298-585e-4c2a-8c0b-7be47347be5e
cris.virtualsource.author-orcid01ff3924-8cb9-41d3-96ac-8f05e3270ebd
cris.virtualsource.author-orcid6452c8a7-a591-4ea2-a747-8a4a33c7581b
cris.virtualsource.author-orcid54c32983-0197-4cba-b58f-e62b74b28281
datacite.rightsopen.access
dc.contributor.authorRicardo-Lax, Inna
dc.contributor.authorLuna, Joseph M
dc.contributor.authorTran, Thi Nhu Thao
dc.contributor.authorLe Pen, Jérémie
dc.contributor.authorYu, Yingpu
dc.contributor.authorHoffmann, H-Heinrich
dc.contributor.authorSchneider, William M
dc.contributor.authorRazooky, Brandon S
dc.contributor.authorFernandez-Martinez, Javier
dc.contributor.authorSchmidt, Fabian
dc.contributor.authorWeisblum, Yiska
dc.contributor.authorTrüeb, Bettina Salome
dc.contributor.authorBerenguer Veiga, Inês Margarida
dc.contributor.authorSchmied, Kimberly
dc.contributor.authorEbert, Nadine
dc.contributor.authorMichailidis, Eleftherios
dc.contributor.authorPeace, Avery
dc.contributor.authorSánchez-Rivera, Francisco J
dc.contributor.authorLowe, Scott W
dc.contributor.authorRout, Michael P
dc.contributor.authorHatziioannou, Theodora
dc.contributor.authorBieniasz, Paul D
dc.contributor.authorPoirier, John T
dc.contributor.authorMacDonald, Margaret R
dc.contributor.authorThiel, Volker Earl
dc.contributor.authorRice, Charles M
dc.date.accessioned2024-10-09T16:52:50Z
dc.date.available2024-10-09T16:52:50Z
dc.date.issued2021-11-26
dc.description.abstractMolecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.
dc.description.numberOfPages8
dc.description.sponsorshipInstitut für Virologie und Immunologie (IVI)
dc.description.sponsorshipInstitut für Tierpathologie (ITPA)
dc.description.sponsorshipDepartment of Infectious Diseases and Pathobiology (DIP)
dc.description.sponsorshipInstitut für Veterinärbakteriologie (IVB)
dc.identifier.doi10.48350/165632
dc.identifier.pmid34648371
dc.identifier.publisherDOI10.1126/science.abj8430
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/67537
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science
dc.relation.ispartofScience
dc.relation.issn0036-8075
dc.relation.organizationDCD5A442C0BAE17DE0405C82790C4DE2
dc.relation.organizationDCD5A442C1CCE17DE0405C82790C4DE2
dc.relation.organizationDCD5A442C494E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442C072E17DE0405C82790C4DE2
dc.relation.schoolDCD5A442C27BE17DE0405C82790C4DE2
dc.subject.ddc500 - Science
dc.subject.ddc500 - Science::570 - Life sciences; biology
dc.subject.ddc500 - Science::590 - Animals (Zoology)
dc.subject.ddc600 - Technology::610 - Medicine & health
dc.subject.ddc600 - Technology::630 - Agriculture
dc.titleReplication and single-cycle delivery of SARS-CoV-2 replicons.
dc.typearticle
dspace.entity.typePublication
dspace.file.typetext
oaire.citation.endPage1106
oaire.citation.issue6571
oaire.citation.startPage1099
oaire.citation.volume374
oairecerif.author.affiliationInstitut für Virologie und Immunologie (IVI)
oairecerif.author.affiliationInstitut für Veterinärbakteriologie (IVB)
oairecerif.author.affiliationInstitut für Tierpathologie (ITPA)
oairecerif.author.affiliationDepartment of Infectious Diseases and Pathobiology (DIP)
oairecerif.author.affiliationDepartment of Infectious Diseases and Pathobiology (DIP)
oairecerif.author.affiliation2Department of Infectious Diseases and Pathobiology (DIP)
oairecerif.author.affiliation2Institut für Virologie und Immunologie (IVI)
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unibe.date.licenseChanged2022-02-24 14:30:54
unibe.description.ispublishedpub
unibe.eprints.legacyId165632
unibe.journal.abbrevTitleSCIENCE
unibe.refereedtrue
unibe.subtype.articlejournal

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