Publication:
Species Detection within the Echinococcus granulosus sensu lato Complex by Novel Probe-Based Real-Time PCRs.

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cris.virtual.author-orcid0000-0003-0782-3723
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dc.contributor.authorMaksimov, Pavlo
dc.contributor.authorBergmann, Hannes
dc.contributor.authorWassermann, Marion
dc.contributor.authorRomig, Thomas
dc.contributor.authorGottstein, Bruno
dc.contributor.authorCasulli, Adriano
dc.contributor.authorConraths, Franz J
dc.date.accessioned2024-09-21T06:11:30Z
dc.date.available2024-09-21T06:11:30Z
dc.date.issued2020-09-26
dc.description.abstractInfections with eggs of Echinococcus granulosus sensu lato (s.l.) can cause cystic echinococcosis in intermediate host animals and humans. Upon ingestion of viable eggs, oncospheres hatch from the eggs and subsequently develop into fluid-filled larval cysts, most frequently in the liver or the lungs. The slowly growing cysts progressively interfere with organ function. The risk of infection is determined by the host range of the parasite, its pathogenicity and other epidemiologically relevant parameters, which differ significantly among the five species within the E. granulosus s.l. complex. It is therefore essential to diagnose the correct species within E. granulosus s.l. to help understand specific disease epidemiology and to facilitate effective implementation of control measures. For this purpose, simple, fast and cost-effective typing techniques are needed. We developed quantitative real-time polymerase chain reactions (qPCRs) to target polymorphic regions in the mitochondrial genome of E. granulosus s.l. In a single-step typing approach, we distinguished E. granulosus s.l. members in four epidemiologically relevant subgroups. These were E. granulosus sensu stricto, E. equinus, E. ortleppi and the E. canadensis cluster. The technique also allowed identification and differentiation of these species from other Echinococcus or Taenia taxa for samples isolated from cysts or faeces.
dc.description.sponsorshipInstitut für Infektionskrankheiten (IFIK)
dc.identifier.doi10.48350/154118
dc.identifier.pmid32993077
dc.identifier.publisherDOI10.3390/pathogens9100791
dc.identifier.urihttps://boris-portal.unibe.ch/handle/20.500.12422/45485
dc.language.isoen
dc.publisherMDPI AG
dc.relation.ispartofPathogens
dc.relation.issn2076-0817
dc.relation.organizationDCD5A442BD12E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442BFE6E17DE0405C82790C4DE2
dc.relation.organizationDCD5A442C1CCE17DE0405C82790C4DE2
dc.subjectDNA probe Echinococcus granulosus sensu lato species diagnosis real-time polymerase chain reaction
dc.subject.ddc500 - Science::570 - Life sciences; biology
dc.subject.ddc600 - Technology::610 - Medicine & health
dc.subject.ddc600 - Technology::630 - Agriculture
dc.titleSpecies Detection within the Echinococcus granulosus sensu lato Complex by Novel Probe-Based Real-Time PCRs.
dc.typearticle
dspace.entity.typePublication
oaire.citation.issue10
oaire.citation.volume9
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oairecerif.author.affiliationInstitut für Infektionskrankheiten (IFIK)
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oairecerif.author.affiliation2Institut für Parasitologie (IPA)
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unibe.date.licenseChanged2021-03-17 08:56:36
unibe.description.ispublishedpub
unibe.eprints.legacyId154118
unibe.journal.abbrevTitlePathogens
unibe.refereedTRUE
unibe.subtype.articlejournal

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