Natural saliva as an adjuvant in a secondary caries model based on Streptococcus mutans
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BORIS DOI
Publisher DOI
PubMed ID
29614462
Description
OBJECTIVE
Two factors for secondary caries formation were evaluated using an artificial biofilm model, saliva as additive in culture medium and bonding procedures of composite materials for artificial gap creation.
DESIGN
Standardized cavities were prepared in bovine tooth samples (n = 44), treated with two different bonding pretreatments, restored and after artificial ageing incubated with Streptococcus mutans in a Mueller-Hinton-Broth-Sugar medium with or without human saliva for seven days. Secondary caries formation was analyzed using confocal laser scanning microscopy and transversal microradiography.
RESULTS
Lesions were significantly pronounced in groups using saliva, but were not influenced by the bonding pretreatments.
CONCLUSIONS
The results indicate that the addition of saliva, but not the type of bonding procedure influences the outcome in the present biofilm-based secondary caries model.
Two factors for secondary caries formation were evaluated using an artificial biofilm model, saliva as additive in culture medium and bonding procedures of composite materials for artificial gap creation.
DESIGN
Standardized cavities were prepared in bovine tooth samples (n = 44), treated with two different bonding pretreatments, restored and after artificial ageing incubated with Streptococcus mutans in a Mueller-Hinton-Broth-Sugar medium with or without human saliva for seven days. Secondary caries formation was analyzed using confocal laser scanning microscopy and transversal microradiography.
RESULTS
Lesions were significantly pronounced in groups using saliva, but were not influenced by the bonding pretreatments.
CONCLUSIONS
The results indicate that the addition of saliva, but not the type of bonding procedure influences the outcome in the present biofilm-based secondary caries model.
Date of Publication
2018-06
Publication Type
Article
Subject(s)
Keyword(s)
Biofilm model Resin composite Saliva Secondary caries Streptococcus mutans
Language(s)
en
Contributor(s)
Hetrodt, Franziska | |
Lausch, Julian | |
Apel, Christian | |
Conrads, Georg |
Series
Archives of oral biology
Publisher
Elsevier Science
ISSN
0003-9969
Access(Rights)
restricted