Microinjection into Xenopus oocytes
Publisher DOI
Abstract
Microinjection of genetic information in the form of complimentary ribonucleic acid (cRNA) into the Xenopus oocyte leads to the functional expression of the encoded proteins. In this novel environment, exogenous proteins may be functionally characterised under well-defined conditions. Complimentary deoxyribonucleic acid (cDNA) coding for the protein may be mutated, the altered cDNA transcribed and polyadenylated in vitro to the corresponding cRNA and the function of the resulting altered protein is compared with that of the wild-type protein. The oocyte is a major expression system used in membrane protein structure–function studies. It is especially popular for expression of membrane transport proteins such as carriers and ion channels. In another approach, total messenger RNA (mRNA) may be prepared from a tissue of choice, microinjected into oocytes, the encoded functions characterised and the encoding genetic information isolated.
Date Issued
2010
Publication Type
Book Section
Language(s)
en
Additional Credits
Publisher
John Wiley & Sons Ltd
Book Title
Access(Rights)
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