Biosensor-based selective detection of Zika virus specific antibodies in infected individuals.
Publisher DOI
PubMed ID
29751200
Abstract
Zika virus (ZIKV) recently emerged as a global threat subsequent to its global spread because it induces microencephaly and other brain damages in infants born to infected mothers. Epidemiological monitoring of infection has been hampered by the absence of reliable serological tests capable to distinguish between ZIKV and other Flavivirus infections, in particular Dengue virus (DENV). As both viruses are transmitted by the same mosquito-species, their distributions largely overlap and reliable serological distinction between the viruses is essential. Here we develop a novel biosensor which is based on recombinant forms of ZIKV non-structural protein 1 (NS1) and the domain III of the envelope protein (EDIII). Using electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV), we demonstrate that in addition to extremely sensitive detection of ZIKV-specific antibodies in serum and saliva, the biosensor promptly distinguished ZIKV and DENV-specific antibodies. Hence, this novel biosensor allows assessing ZIKV antibodies in blood and saliva and results are unaffected by presence of DENV virus-specific antibodies.
Date Issued
2018-08-15
Publication Type
Article
Subject(s)
Subjects
Antibody detection Biosensor Dengue Zika-specific
Language(s)
en
Author(s)
Cardoso, Ana R | |
Ferreira, Luis C S | |
Sales, M Goreti F |
Additional Credits
Journal
Biosensors & bioelectronics
Publisher
Elsevier
ISSN
0956-5663
Access(Rights)
restricted